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| 利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定 | |
| 引用本文： | 雷云霆,赵闫闫,喻凤,李媛,窦全文.利用基因组SSR分子标记对老芒麦品种(种质)鉴别和品种纯度鉴定[J].草地学报,2015,23(1):151-155. |
| 作者姓名： | 雷云霆 赵闫闫 喻凤 李媛 窦全文 |
| 作者单位： | 1. 中国科学院西北高原生物研究所, 青海 西宁 810001; 2. 中国科学院大学, 北京 100049 |
| 基金项目： | 青海省科技厅科技促进新农村建设计划项目，中科院“西部之光”联合学者人才培养计划项目资助 |
| 摘 要： | 利用从老芒麦(Elymus sibiricus)自身基因组中开发出来的6对多态性SSR引物,对3个老芒麦牧草品种和7个种质进行分子指纹图谱鉴别研究.结果表明:6对SSR引物在这10个材料中共检测出21个多态性SSR标记片段,每对引物可以检测到2~5个数目不等的片段,平均为3.5个.6对引物中3对核心引物组合可以将这10个材料进行有效鉴别.进一步选用3对引物对2个老芒麦品种进行纯度鉴定,表明SSR标记可以对品种群体中具有变异位点的个体进行有效鉴定,同时揭示野生栽培品种‘同德’老芒麦比育成品种‘多叶'老芒麦具有较高的遗传变异度. |
| 关 键 词： | 老芒麦 基因组SSR分子标记 品种(种质)鉴别 纯度鉴定 |
| 收稿时间： | 2014-02-27 |
Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers |
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| LEI Yun-ting,ZHAO Yan-yan,YU Feng,LI Yuan,DOU Quan-wen.Cultivars (Grmplasms) Discrimination and Seed Purity Assessment of Grass Elymus sibiricus Using Genomic SSR Markers[J].Acta Agrestia Sinica,2015,23(1):151-155. | |
| Authors: | LEI Yun-ting ZHAO Yan-yan YU Feng LI Yuan DOU Quan-wen |
| Institution: | 1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, Chian; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
| Abstract: | Molecular fingerprinting maps of 3 Elymus sibiricus cultivars and 7 germplasms were carried out using 6 simple sequences repeat (SSR) markers developed from the genomic DNA of E. sibiricus. A total of 21 polymorphic SSR fragments were detected in all the tested materials, and 2 to 5 alleles were detected by each pair of SSR primer with an average of 3.5. All the 3 cultivars and 7 germplasms could be well distinguished by the fingerprinting maps constructed by 3 pairs of core primers. In addition, seed purity assessment was conducted in cultivars ‘Tongde' and ‘Duoye' with 3 pairs of SSR primers. It showed that seed purity and the polymorphism of SSR alleles could be effectively identified. Moreover, it revealed that ‘Tongde', a domesticated cultivar, had much lower purity and higher polymorphic alleles than ‘Duoye', a breeding cultivar. The SSR markers used in this study can be a powerful tool for cultivars or germplasms identification, seeds purity assessment in E. sibiricus. |
| Keywords: | Elymus sibiricus Genomic SSR markers Cultivar discrimination Cultivar purity assessment |
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