| 不同染色剂对SDS-PAGE染色体系的影响 |
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| 引用本文： | 林方养,杨耀东,万婕,高丽娜,马子龙,吴翼.不同染色剂对SDS-PAGE染色体系的影响[J].安徽农业科学,2014(9):2562+2610-2562,2610. |
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| 作者姓名： | 林方养 杨耀东 万婕 高丽娜 马子龙 吴翼 |
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| 作者单位： | 中国热带农业科学院椰子研究所,海南省热带油料作物生物学重点实验室,海南文昌571339;琼州学院,海南五指山,572200;中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 海南省自然科学基金(312039) |
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| 摘 要： | 目的]研究不同染色剂对SDS-PAGE染色体系的影响.方法]通过SDS-PAGE染色体系中不同考马斯亮蓝染料、染色方法和染色时间的比较,探讨适合油棕和椰子的最佳染色体系.结果]改进后的方法染色效果好,较好脱色,凝胶背景清晰,容易分析蛋白含量较低的条带.染色时间短（2 h）,条带较少,一些低丰度的蛋白条带未能清晰地显示出来,适当延长染色时间（6h或过夜）效果较好.3种不同染料均能较好地对蛋白质条带染色,R350和R250相比G250效果稍好些,条带着色深,蛋白质含量低的细小条带也能较好的染色.结论]SDS-PAGE是开展蛋白质组学研究的关键技术,建立简易、稳定的染色方法具有重要意义.
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| 关 键 词： | SDS-PAGE 考马斯亮蓝 染色 |
Effects of Different Dyes on SDS-PAGE Gel Staining System |
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| LIN Fang-yang,WU Yi.Effects of Different Dyes on SDS-PAGE Gel Staining System[J].Journal of Anhui Agricultural Sciences,2014(9):2562+2610-2562,2610. |
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| Authors: | LIN Fang-yang WU Yi |
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| Institution: | et al ( Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan 571339) |
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| Abstract: | Objective] To study effects of different dyes on SDS-PAGE gel staining system. Method] Different SDS-PAGE staining methods with different Coomassie Brilliant Blue dye and staining time were compared to explore suitable staining system for oil palm and coconut SDSPAGE study. Result] The improved method of dyeing effect is good, good decolorization, gel background is clear, easy to analysis with low protein content. Short dyeing time （2 h）, with less band, some low abundance protein band failed to show, prolonged dyeing time （6 h or overnight） would be better. Conclusion] SDS-PAGE is a key technology to carry out proteomic studies, the establishment of a simple, stable staining method is important. |
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| Keywords: | SDS-PAGE Coomassie blue Staining |
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