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| HPLC和SOS显色反应检测不同微生物脱除4-NQO基因毒性能力 | |
| 引用本文： | 胡明臣,任发政,王芳,张明,邱芳萍.HPLC和SOS显色反应检测不同微生物脱除4-NQO基因毒性能力[J].乳业科学与技术,2011,34(5):207-210. |
| 作者姓名： | 胡明臣 任发政 王芳 张明 邱芳萍 |
| 作者单位： | 1. 长春工业大学化学与生命科学学院,吉林长春,130012 2. 中国农业大学食品科学与营养工程学院,北京,100083 3. 北京农业职业学院食品与生物工程系,北京,102442 |
| 摘 要： | 应用高效液相色谱法(high performance liquid chromatography,HPLC)和SOS显色反应测定36株益生菌脱除4-硝基喹啉-1-氧化物(4-NQO)基因毒性的能力,同时用HPLC对它们的图谱进行比较分析。研究发现,基因毒性清除率高的菌株有1株植物乳杆菌、5株双歧杆菌、干酪乳杆菌代田株、3株芽孢杆菌、2株酵母菌、7株唾液乳杆菌和一株保加利亚乳杆菌。同属于一个菌属的各个菌株基因毒性清除率之间差异不显著。图谱分析发现,只有唾液乳杆菌才产生P1,即益生菌脱除4-NQO基因毒性具有菌株特异性。研究还发现益生菌转化4-NQO为4-HAQO的能力是决定益生菌脱除4-NQO基因毒性能力大小的关键。 |
| 关 键 词： | 基因毒性 SOS显色反应 高效液相色谱法(HPLC) 菌株特异性 |
Determination of Antigenotoxic Activity of Different Probiotic Microorganisms against 4-Nitroquino-line-l-oxide （4-NQO） by SOS Chromotest and HPLC |
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| HU Ming-chen,REN Fa-zheng,WANG Fang,ZHANG Ming,QIU Fang-ping.Determination of Antigenotoxic Activity of Different Probiotic Microorganisms against 4-Nitroquino-line-l-oxide （4-NQO） by SOS Chromotest and HPLC[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2011,34(5):207-210. | |
| Authors: | HU Ming-chen REN Fa-zheng WANG Fang ZHANG Ming QIU Fang-ping |
| Institution: | 1. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China ; 2. College of Food Science and Nutritional Engineering, China Agricltural University, Beijing 100083, China; 3. School of Food and Biological Engineering, Beijing Agricltural Vocational University, Beijing 102442, China) |
| Abstract: | SOS chromotest and HPLC were used for determining the antigenotoxic activity of 36 probiotic strains against 4-NQO. Meanwhile, their ability to remove 4-NQO genotoxicity was comparatively analyzed by HPLC. One L. plantarum strain, 5 Bifidobacterium strains, L. casei strain Shirota, 3 Bacillus strains, 2 yeast strains, 7 Lsalivarius strains and I Lactobacillus bulgaricus strain were found to have a higher ability to remove 4-NQO genotoxicity among these strains. No significant difference in removal ability for 4-NQO genotoxicity was observed among strains from the same genus. Only L.salivarius strains could produce Px, suggesting the species-specific removal ability of probiotics for 4-NQO genotoxicity. Also, we found that the capability of converting 4-NQO into 4-HAQO decided the ability of probiotics to remove 4-NQO genotoxicity. This study can provide references and supports for further studies of the removal mechanism of 4-NQO genotoxicity by probiotics. |
| Keywords: | genotoxicity SOS Chromotest HPLC species specificity |
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