| 苏云金杆菌深层发酵补料分批培养工艺研究 |
| |
| 引用本文： | 李世杰,方尚玲,刘华梅,王志林.苏云金杆菌深层发酵补料分批培养工艺研究[J].湖北农业科学,2001,1(1):39-41. |
| |
| 作者姓名： | 李世杰 方尚玲 刘华梅 王志林 |
| |
| 作者单位： | 1. 湖北工学院生物工程系武汉430068
2. 湖北省农业科学院生物农药工程中心 |
| |
| 摘 要： | 补料培养与分批培养进行平行对比实验结果 ,补料培养相对于分批培养 ,培养过程Bt最大菌数平均提高了 4 0亿 /ml,晶体含量平均提高了 0 83mg/ml,毒力效价平均提高 1 80 0IU/μl。且补料工艺对生长高峰期溶氧的改善有明显的效果。
|
| 关 键 词： | 苏云金杆菌(Bt) 通风发酵 高密度 补料分批培养 |
| 修稿时间： | 2000年9月28日 |
Study on the feed batch culture technology of Bt submerged fermentation |
| |
| Li Shijie.Study on the feed batch culture technology of Bt submerged fermentation[J].Hubei Agricultural Sciences,2001,1(1):39-41. |
| |
| Authors: | Li Shijie |
| |
| Abstract: | Using B. Brawn 10 litter automatic fermentor, Bt submergedfermentation was held to study the characteristics of Feed Batch culture process. With Feed batch technology, the fermentation broth dissolved oxygen (DO) was improved notable. Compare to batch culture, the cell density, crystal concentration and bioassay efficacy were increased by an average of 4.0 × 109/ml, 0.83mg/ml and 1800 IU/μl respectively. |
| |
| Keywords: | Bacillus thuringiensis(Bt) aerated fermentation feed batch culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
