| CFNCpG对BVDV1灭活抗原的免疫佐剂效应研究 |
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| 引用本文： | 刘艳环,王志刚,李海涛,李庆超,张广雷,苗利光.CFNCpG对BVDV1灭活抗原的免疫佐剂效应研究[J].特产研究,2008,30(4). |
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| 作者姓名： | 刘艳环 王志刚 李海涛 李庆超 张广雷 苗利光 |
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| 作者单位： | 中国农业科学院特产研究所,吉林,吉林,132109 |
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| 基金项目： | 中国农业科学院特产研究所科研基金项目
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| 摘 要： | 分别用CFNCpG、弗氏完全佐剂、氢氧化铝胶与BVDV1灭活抗原配制成疫苗免疫家兔,于免疫后每间隔7d检测中和抗体变化,来探讨CFNCpG对BVDV1灭活抗原的佐剂效应。试验结果表明,CFNCpG单独作为佐剂应用7d可使抗体滴度达到4.4(log_2SN_(50)),14d可达到7.0以上;与氢氧化铝胶联合可使抗体滴度在21d达到7.0以上,其佐剂水平与弗氏完全佐剂相当。有望进—步研制成为BVDV1灭活疫苗佐剂成分。
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| 关 键 词： | 佐剂 |
Immune Adjuvant Effect of CFNCpG Motif on Antigen of Inactivated BVDV1 |
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| LIU Yan-huan,WANG Zhi-gang,LI Hai-tao,LI Qing-chao,ZHANG Guang-lei,MIAO Li-guang.Immune Adjuvant Effect of CFNCpG Motif on Antigen of Inactivated BVDV1[J].Special Wild Economic Animal and Plant Research,2008,30(4). |
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| Authors: | LIU Yan-huan WANG Zhi-gang LI Hai-tao LI Qing-chao ZHANG Guang-lei MIAO Li-guang |
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| Institution: | LIU Yan-huan WANG Zhi-gang LI Hai-tao LI Qing-chao ZHANG Guang-lei MIAO Li-guang~* |
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| Abstract: | The adjuvant effects of CFNCpG on the humoral immune response of rabbits were investigated on the CFNCpG,Freund's complete adjuvant and aluminium hydroxide.An inactivated BVDV-1 was served as antigen.The neutralizing antibody titers were tested every 7 days after immunization to evaluate the immune adjuvant effects of CFNCpG on antigen of inactivated BVDV-1.The results showed that the antibody titer was 4.4(log_2SN_)(50))7 days and more than 7.0 14 days after CFNCpG was used as adjuvant alone. When CFNCpG combined with aluminium hydroxide was used as adjuvants,the antibody titer was higher than 7.0 after 21 days which was equal to the adjuvant effects of Freund's complete adjuvant.The CFNCpG is a useful adjuvant component in BVDV-1 inactivated vaccine in further research. |
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| Keywords: | BVDV1 CFNCpG |
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