| 稻米直链淀粉含量基因座位的分子标记定位 |
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| 引用本文： | 黄祖六,谭学林,Tragoonrung S,Vanavichit A.稻米直链淀粉含量基因座位的分子标记定位[J].作物学报,2000,26(6):777-782. |
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| 作者姓名： | 黄祖六 谭学林 Tragoonrung S Vanavichit A |
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| 作者单位： | 扬州大学农学院数量遗传研究室， 江苏扬州 225009 |
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| 基金项目： | 洛克菲勒基金项目，国家自然科学基金项目! (批准号 :3990 0 0 80 )资助 |
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| 摘 要： | 以直链淀粉含量(AC)中等的CT9993和泰国优质的香软米KDML105杂交产生的152个重组近交系 (RIL)为材料， 构建了含83个RFLP、 69个AFLP和15个微卫星(SSLP)标记的分子标记连锁图 ， 标记间平均距离为12.98 cM。 应用该连锁图对控制稻米AC的基因座位(QTL)进行了分析 。 结果表明： 稻米AC主要受两个主效QTL和5个微效QTL的共同控制
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| 关 键 词： | 稻米品质 直链淀粉含量(AC) 数量性状基因座位(QTL) 基因定 位 RFLP标记 AFLP标记 微卫星标记(SSLP) |
| 收稿时间： | 1999-05-18 |
| 修稿时间： | 1999-05-18 |
Mapping QTLs for Amylose Content of Grain with Molecular Markers in Rice (Oryza sativa L.) |
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| Tragoonrung S,Vanavichit A.Mapping QTLs for Amylose Content of Grain with Molecular Markers in Rice (Oryza sativa L.)[J].Acta Agronomica Sinica,2000,26(6):777-782. |
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| Authors: | Tragoonrung S Vanavichit A |
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| Institution: | Lab of Quantitative Genetics, Agric Coll, Yangzhou University, Ya ngzhou, Ji angsu 225009 |
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| Abstract: | A population of 152 recombinant inbred lines (RILs) derived from a cross between CT9993, a japonica rice with intermediate amylose content (AC), and Khoa Dawk M ali105( KDML 105 ), the famous indica quality rice with low AC, was used for m apping quantitative trait loci (QTLs) underlying AC with molecular markers. The linkage map was constructed from 83 RFLP, 69 AFLP and 15 SSLP markers, with an a verage distance of 12.98 cM between markers. Based on the map, QTLs conferring A C were analyzed. The result showed that AC is mainly controlled by two major QTL s located on chromosome 3 and 6 as well as five minor QTLs, the major QTLs were detected with LOD scores more than 8.0, and one on chromosome 3 is linked with R FLP marker R2170 and another on chromosome 6 is linked to R1962 that is closed t o wx locus. According to the analysis of variation of AC for RILs, there is no interaction between the two major loci. The key points for mapping QTLs in th e biased population are discussed. |
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| Keywords: | Quality trait of rice Amylose content (AC) Quantitative trait locus(QTL) Gene mapping RFLP AFLP Microsatellite(SSLP) |
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