|
|||||
|
|
Occurrence of fatal canine Angiostrongylus vasorum infection in Italy |
|
| Authors: | Traversa Donato Torbidone Alessandra Malatesta Daniela Guglielmini Carlo |
| Institution: | aThe College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China bNational Institute for Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, PR China cDivision of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, GA 30341, USA |
| Abstract: | A total of 469 fecal samples were collected from American minks (Mustela vison) on a farm in Hebei Province in China and examined for Cryptosporidium by Sheather's sugar flotation technique and 8 Cryptosporidim isolates were obtained. The partial 18S rRNA, 70 kDa heat shock protein (HSP70), Cryptosporidium oocyst wall protein (COWP) and actin genes of six isolates were sequenced. Sequence data were analyzed together with known Cryptosporidium spp. and genotypes. Results of this multi-locus genetic characterization indicated that the six Cryptosporidium isolates in this study shared the same sequences of the genes studied and were different from known Cryptosporidium species and genotypes. The closest relative was Cryptosporidium ferret genotype with 7, 22, 2 and 2 nucleotide differences in the 18S rRNA, HSP70, COWP and actin genes, respectively. The homology to ferret genotype at the 18S rRNA locus was 99.1%, which is comparable to that between C. parvum and C. hominis (99.2%), or between C. muris and C. andersoni (99.4%). Therefore, the Cryptosporidium in minks in this study is considered a new genotype, the Cryptosporidium mink genotype. |
| Keywords: | Cryptosporidium Mink Mink genotype 18S rRNA HSP70 COWP Actin |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |