| 利用家蚕生产慈姑蛋白酶抑制剂 |
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| 引用本文： | 季平,张志芳,何家禄,宓怡德,吴祥甫.利用家蚕生产慈姑蛋白酶抑制剂[J].蚕业科学,1995,21(4):223-227. |
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| 作者姓名： | 季平 张志芳 何家禄 宓怡德 吴祥甫 |
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| 作者单位： | 中国农业科学院蚕业研究所，中国科学院上海生物化学研究所 |
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| 摘 要： | 将慈姑蛋白酶抑制剂Ｂ（ＡｒｒｏｗｈｅａｄＰｒｏｔｅｉｎａｓｅＩｎｈｉｂｉｔｏｒ，Ｂ，ＡＩＢ）基因插入转移载体ｐＵＢＭ—４中，得到重组转移载体ｐＵＢＭ（ＡＩＢ）；与Ｂｍ—ＮＰＶＤＮＡ共转染家蚕细胞，经空斑纯化，得到不含多角体的重组病毒ＢｍＮＰＶ（ＡＩＢ）；感染家蚕幼虫，利用家蚕高效表达了ＡＩＢ，其表达量为１．０×１０５Ｕ／ｍＬ血淋巴，比活为３．２９×１０３Ｕ／ｍｇ。
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| 关 键 词： | 慈姑蛋白酶抑制剂B，cDNA序列，家蚕核型多角体病毒，空斑纯化，基因表达 |
PRODUCTION OF THE ARROWHEAD PROTEINASE INHIBITOR IN THE SILKWORM LARVAE |
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| Ji Ping Zhang ZHifang He Jialu.PRODUCTION OF THE ARROWHEAD PROTEINASE INHIBITOR IN THE SILKWORM LARVAE[J].Acta Sericologica Sinica,1995,21(4):223-227. |
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| Authors: | Ji Ping Zhang ZHifang He Jialu |
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| Abstract: | Arrowhead proteinase inhibitor B sequence was cloned into transfer vector pUBM 4 to form the recombinant transfer vector pUBM(AIB).Then ,pUBM(AIB)and BmNPV DNA were co-transfected into the BmN with lipofectin entrapment. After that,the recombinant virus of BmNPV(AIB)Ⅰ Ⅲ were isolated and purified by plaque assay.AIB was expressed in the silkworm larvae at high level. The product exhibited bilogical activity. |
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| Keywords: | Arrowhead proteinase inhibitor B cDNA sequence BmNPV Plaque assay Gene expression |
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