The humoral immune response to recombinant nucleocapsid antigen of canine distemper virus in dogs vaccinated with attenuated distemper virus or DNA encoding the nucleocapsid of wild-type virus |
| |
| Authors: | Griot-Wenk M E Cherpillod P Koch A Zurbriggen R Bruckner L Wittek R Zurbriggen A |
| |
| Institution: | Institute of Animal Neurology, Bremgartenstrasse 109a, University of Berne, 3012 Berne, Switzerland. mgriot-wenk@healthecon.com |
| |
| Abstract: | This study compared the humoral immune response against the nucleocapsid-(N) protein of canine distemper virus (CDV) of dogs vaccinated with a multivalent vaccine against parvo-, adeno-, and parainfluenza virus and leptospira combined with either the attenuated CDV Onderstepoort strain (n = 15) or an expression plasmid containing the N-gene of CDV (n = 30). The vaccinations were applied intramuscularly three times at 2-week intervals beginning at the age of 6 weeks. None of the pre-immune sera recognized the recombinant N-protein, confirming the lack of maternal antibodies at this age. Immunization with DNA vaccine for CDV resulted in positive serum N-specific IgG response. However, their IgG (and IgA) titres were lower than those of CDV-vaccinated dogs. Likewise, DNA-vaccinated dogs did not show an IgM peak. There was no increase in N-specific serum IgE titres in either group. Serum titres to the other multivalent vaccine components were similar in both groups. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
