| 三黄鸡ST3Gal6基因的半定量RT-PCR及生物信息学分析 |
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| 引用本文： | 周飞,崔连成,安玉甫,宁章勇.三黄鸡ST3Gal6基因的半定量RT-PCR及生物信息学分析[J].中国畜牧兽医,2012,39(3):51-54. |
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| 作者姓名： | 周飞 崔连成 安玉甫 宁章勇 |
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| 作者单位： | 华南农业大学兽医学院病理教研室,广东广州 510642 |
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| 摘 要： | 本研究旨在对三黄鸡ST3Gal6基因进行组织表达谱和生物信息学分析。参考三黄鸡ST3Gal6基因序列设计引物,采用PCR技术克隆三黄鸡ST3Gal6基因序列,并利用半定量RT-PCR进行组织表达谱分析;同时对其进行生物信息学分析。结果表明,克隆的三黄鸡ST3Gal6基因全长1169 bp,含有1059 bp的完整CDS编码区,编码352个氨基酸。其CDS编码区的核苷酸序列与人、黑猩猩、牛、大鼠、蟾ST3Gal6基因对应序列的同源性分别为62%、62%、61.9%、59%、54.4%。组织表达谱分析表明,ST3Gal6基因在各组织均不同程度地表达,其中在大脑表达量很高,肺脏中最低。生物信息学预测ST3Gal6蛋白结构发现,三黄鸡的ST3Gal6蛋白存在2个跨膜螺旋结构域,同时预测ST3Gal6存在22个磷酸化位点和1个特异性蛋白激酶磷酸化位点。
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| 关 键 词： | 唾液酸转移酶 流感受体 鸡 半定量 生物信息学 |
| 收稿时间： | 2011-09-02 |
Semi-quantitative RT-PCR and Bioinformatics Analysis of Sanhuang Chicken ST3Gal6 Gene |
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| ZHOU Fei
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CUI Lian-cheng
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AN Yu-fu
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NING Zhang-yong.Semi-quantitative RT-PCR and Bioinformatics Analysis of Sanhuang Chicken ST3Gal6 Gene[J].China Animal Husbandry & Veterinary Medicine,2012,39(3):51-54. |
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| Authors: | ZHOU Fei CUI Lian-cheng AN Yu-fu NING Zhang-yong |
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| Institution: | College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | This study was designed to analyse the tissue specific expression profile and bioinformatics characteristic of ST3Gal6 in Chinese yellow chicken.Based on the sequence of the ST3Gal6 of the chicken reported in NCBI,2 pairs of primers were designed and the primers were used to amplify the Chinese yellow chicken ST3Gal6 gene by PCR.Then the tissue specific expression profile was analyzed by semi quantitative RT-PCR,and the bioinformatics analysis of ST3Gal6 was summarized.The results showed that,the full length of the cloning gene of ST3Gal6 was 1169 bp,including the 1059 bp of CDS sequence of chicken ST3Gal6,which encoding a 352 amino acid residues deduced protein.This nucleotide sequence of CDS shared 62%,62%,61.9%,59%,54.4% homology with the ST3Gal6 mRNA from human,pongo pygmaeus,cattle,mouse,Xenopus laevis respectively.Semi quantitative PCR revealed that the ST3Gal6 gene was expressed in various tissues,but at different levels.The expression level of this gene was higher in the brain while lower in lung tissue.The biologic softwares were used to predict the bioinformatics of ST3Gal6,which showed that the ST3Gal6 was a kind of transmembrance protein and have 2 transmembrane domains in chicken.There were 22 phosphorylattion sites and one phosphory lation site of specific protein kinase in ST3Gal6 protein. |
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| Keywords: | sialyltransferase influenza receptor chicken semi quantitative RT-PCR bioinformatics |
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