| 猪孤雌激活早期胚胎线粒体分布及mtDNA拷贝数变化的研究 |
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| 引用本文： | 成文敏,魏红江,潘伟荣,信吉阁,黄言,曾养志.猪孤雌激活早期胚胎线粒体分布及mtDNA拷贝数变化的研究[J].中国畜牧兽医,2012,39(1):127-131. |
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| 作者姓名： | 成文敏 魏红江 潘伟荣 信吉阁 黄言 曾养志 |
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| 作者单位： | 云南农业大学动物科学技术学院,云南省版纳微型猪近交系重点实验室,云南昆明650201 |
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| 基金项目： | 国家自然科学基金，云南省教育厅科学研究基金重点项目 |
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| 摘 要： | 本研究通过线粒体分子探针标记技术检测孤雌激活早期胚胎线粒体的分布变化,运用实时荧光定量PCR技术检测mtDNA拷贝数的变化,揭示早期胚胎发育过程中线粒体分布、mtDNA拷贝数变化趋势。结果表明,成熟卵母细胞电激活后,由2-细胞胚胎开始,卵裂球内线粒体分布均匀且密集,每个细胞均有分布,卵裂球之外的空隙未见线粒体分布,直到囊胚形成,线粒体均有分布。孤雌激活4-细胞胚胎mtDNA拷贝数显著高于8-细胞胚胎mtDNA拷贝数(907210.77±145520.77,186224.33±103308.00,P<0.05),但显著低于2-细胞胚胎、桑椹胚、囊胚的mtDNA拷贝数(1563422.54±224666.51、1697626.25±176999.53和1752301.29±101146.64,P<0.05)。孤雌激活扩张囊胚mtDNA拷贝数最高,为2812545.67±156819.31,显著高于其他发育时期胚胎的mtDNA拷贝数(P<0.05)。由此可见,孤雌激活早期胚胎发育进程中线粒体分布及mtDNA拷贝数会发生变化。
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| 关 键 词： | 猪卵母细胞 孤雌激活 线粒体分布 mtDNA拷贝数 |
| 收稿时间： | 2011-09-28 |
Mitochondrial Distribution and mtDNA Copy Number of Porcine Parthenogenetic Embryos |
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| CHENG Wen-min
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WEI Hong-jiang
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PAN Wei-rong
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XIN Ji-ge
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HUANG Yan
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ZENG Yang-zhi.Mitochondrial Distribution and mtDNA Copy Number of Porcine Parthenogenetic Embryos[J].China Animal Husbandry & Veterinary Medicine,2012,39(1):127-131. |
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| Authors: | CHENG Wen-min WEI Hong-jiang PAN Wei-rong XIN Ji-ge HUANG Yan ZENG Yang-zhi |
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| Institution: | Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Mito-Tracker Green and Real-time quantitative PCR were used to respectively detect the mitochondria distribution and mtDNA copy number of porcine parthenogenetic oocytes.The results showed that the distribution of mitochondria was uniform and dense in the every blastomere beginning from 2-cell embryo.The outside of the space in blastomeres was no distribution of mitochondria.Until the blastocyst formation,mitochondria were distributed in blastomeres.mtDNA copy number of 4-cell embryos was significantly higher than that of 8-cell embryos(907210.77±145520.77,186224.33±103308.00,P<0.05),and it was significantly lower than those of 2-cell embryos,molura and blastocyst(1563422.54±224666.51,1697626.25±176999.53 and 1752301.29±101146.64,P<0.05).mtDNA copy number of parthenogenetic expanded blastocyst was 2812545.67±156819.31,which was the highest value of all different developmental embryos.In conclusion,mitochondrial distribution and mtDNA copy number changes could differ in the developmental process of parthenogenetic embryos. |
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| Keywords: | porcine oocyte parthenogenetic mitochondrial distribution mtDNA copy number |
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