| 鸭肝炎病毒GD株的分离与RT-PCR鉴定分析 |
| |
| 引用本文： | 黄昌力,魏春娅,张超轶,袁镜乐,刘志彬,张桂红.鸭肝炎病毒GD株的分离与RT-PCR鉴定分析[J].中国畜牧兽医,2012,39(2):156-159. |
| |
| 作者姓名： | 黄昌力 魏春娅 张超轶 袁镜乐 刘志彬 张桂红 |
| |
| 作者单位： | 华南农业大学兽医学院,广东广州 510642 |
| |
| 摘 要： | 本研究分离了1株鸭肝炎病毒(duck hepatitis virus,DHV),并对其进行了分型研究。取广东某鸭场疑似DHV致死8日龄雏鸭肝脏,肝脏组织处理后经鸭胚接种分离纯化病毒。分离毒株经动物回归试验、ELD50测定、中和试验等确定为血清Ⅰ型DHV,同时通过RT-PCR检测及序列分析,从基因分型角度也验证分离毒株为DHV-Ⅰ,并将该分离毒命名为GD株。
|
| 关 键 词： | 鸭肝炎病毒 分离 RT-PCR 鉴定分析 |
| 收稿时间： | 2011-08-22 |
Isolation and Identification Analysis of Duck Hepatitis Virus GD Strain by RT-PCR |
| |
| HUANG Chang-li
,
WEI Chun-ya
,
ZHANG Chao-yi
,
YUAN Jing-le
,
LIU Zhi-bin
,
ZHANG Gui-hong.Isolation and Identification Analysis of Duck Hepatitis Virus GD Strain by RT-PCR[J].China Animal Husbandry & Veterinary Medicine,2012,39(2):156-159. |
| |
| Authors: | HUANG Chang-li WEI Chun-ya ZHANG Chao-yi YUAN Jing-le LIU Zhi-bin ZHANG Gui-hong |
| |
| Institution: | College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China |
| |
| Abstract: | In order to complete a duck hepatitis virus(DHV) stain’s isolation and sero-genotyping research,the suspected DHV liver from died duckling in Guangdong province was selected as sample,and then was carried on regression analysis,ELD50 determination and duck embryo neutralization test after viral isolation,duck embryo inoculation and purification.The results showed that the serotype of the duck hepatitis virus was typeⅠ.According to RT-PCR detect and sequence analysis,it was also identified as genetype Ⅰ of DHV.Named the isolated virus strain as GD. |
| |
| Keywords: | duck hepatitis virus isolation RT-PCR identification analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
