| 青海地区奶牛无乳链球菌的分离与鉴定 |
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| 引用本文： | 杨有武.青海地区奶牛无乳链球菌的分离与鉴定[J].中国畜牧兽医,2012,39(2):191-193. |
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| 作者姓名： | 杨有武 |
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| 作者单位： | 青海省海北州海晏县兽医站,青海海北,812200 |
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| 摘 要： | 无乳链球菌是导致奶牛乳房炎的常见病原菌之一,为了掌握青海地区无乳链球菌的流行情况及常用药物的敏感性,从而为本地区更好的预防与治疗奶牛乳房炎提供保障。本试验在青海地区16个奶牛场共采集258份乳房炎乳样,经革兰氏染色与绵羊鲜血琼脂培养基培养纯化,获得疑似链球菌137株;随后经玻片法血浆凝固酶试验、CAMP试验、生化试验与动物致病性试验对无乳链球菌进行鉴定,结果显示,137株链球菌中含有无乳链球菌42株,分离率为30.7%;经K-B纸片扩散法对无乳链球菌耐药性检测结果显示,该地区无乳链球菌主要对头孢类高度敏感,对氧氟沙星、磺胺类、氨苄青霉素与阿米卡星中度敏感。
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| 关 键 词： | 奶牛 乳房炎 无乳链球菌 分离鉴定 |
| 收稿时间： | 2011-06-02 |
Isolation and Identification of Streptococcus agalactiae from Dairy Cow Mastitis in Qinghai Province |
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| YANG You-wu.Isolation and Identification of Streptococcus agalactiae from Dairy Cow Mastitis in Qinghai Province[J].China Animal Husbandry & Veterinary Medicine,2012,39(2):191-193. |
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| Authors: | YANG You-wu |
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| Institution: | Haiyan County Veterinary Station of Qinghai Province, Haibei 812200, China |
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| Abstract: | Streptococcus agalactiae is one of pathogenic bacterium that inducing mastitis of dairy cow.In order to master the popularity and laid the reference for selection of medicine to treat mastitis,258 samples were collected from 16 dairy cow farms in Qinghai province,and 137 Streptococcus strains were isolated,including Streptococcus agalactiae 42 strains after sub-culture,gram straining,plasma-coagulase test,CAMP test,biochemical tests and animal pathogenicity tests.The results indicated that Streptococcus agalactiae was the primary pathogenic bacteria of mastitis.All of Streptococcus agalactiaes were hypersensitive to cephradine,and partially resistant to amikacin ofloxacin,ampicillin and sulfanilamide group after K-B medicine sensitivity test. |
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| Keywords: | dairy cow mastitis Streptococcus agalactiae isolation and identification |
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