| 泥鳅消化道6种内分泌细胞的免疫组织化学定位 |
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| 引用本文： | 金显文,黄山,吴孝兵.泥鳅消化道6种内分泌细胞的免疫组织化学定位[J].农业科学与技术,2009,10(1):135-139. |
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| 作者姓名： | 金显文 黄山 吴孝兵 |
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| 作者单位： | 金显文,JIN Xian-wen(淮北煤炭师范学院生命科学学院,安徽淮北,235000);黄山,HUANG Shan(淮北煤炭师范学院体育学院,安徽淮北,235000);吴孝兵,WU Xiao-bing(安徽师范大学生命科学学院,安徽芜湖,241000)
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| 基金项目： | 资源植物生物学安徽省重点实验室项目 |
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| 摘 要： | 1材料与方法
1．1材料泥鳅4尾，雌雄各半，2007年5月购自安徽省芜湖吉合菜市场。置于清水中暂养24h后迅速解剖，按下列消化道各段取材：食道、前肠前段、前肠后段、中肠、后肠前段、直肠。生理盐水洗净，用无冰醋酸的Bouin氏液固定18—24h，常规脱水透明，石蜡包埋，切片5～6μm，贴片，37℃温箱烘干备用。
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| 关 键 词： | 免疫组织化学定位 消化道 内分泌细胞 泥鳅 生理盐水 石蜡包埋 菜市场 安徽省 |
Immunohistochemical Location of Six Kinds of Endocrine Cell from Digestive Tract of Misgurnus anguillicaudatus |
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| JIN Xian-wen,HUANG Shan,WU Xiao-bing.Immunohistochemical Location of Six Kinds of Endocrine Cell from Digestive Tract of Misgurnus anguillicaudatus[J].Agricultural Science ＆ Technology,2009,10(1):135-139. |
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| Authors: | JIN Xian-wen HUANG Shan WU Xiao-bing |
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| Institution: | 1. School of Life Science, Huaibei Coal Industry Teachers College, Huaibei 235000; 2. Institute of Physical Education, Huaibei Coal Industry Teacher College,Huaibei 235000; 3. College of Life Science,Auhui Normal University, Wuhu 241000) |
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| Abstract: | Objective] The study was to immunohistochemical localize the endocrine cells from digestive tract of Misgurnus anguillicaudatus.Method] Using six gastrointestinal hormone antisera, the endocrine cells from digestive tract of M.anguillicaudatus was immunohistochemical localized.Result] The 5-hydroxytryptamine immunoreactive(5-HT-IR) cells distribute in oesophagus, foregut and midgut; the distribution density was determined to be forepart of foregut> oesophagus and hindpart of foregut>gut, and the differences in the three density gradients reached significant level. Like PP-IR, SS-IR cells were observed mostly in oesophagus, followed by hindpart of foregut, least in forepart of foregut, but never found in gut and hindgut. The three kinds of immunocompetent cells Gas-IR, Glu-IR and SP-IR were not detected in each part of digestive tract. Conclusion] This study may provide basic data for studying the nutritional and digestive physiology, as well as the preparation of meridic diets for M.anguillicaudatus. |
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| Keywords: | Misgurnus anguillicaudatus Cantor Digestive tract Endocrine cells Immunohistochemistry |
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