| 水稻品种对褐飞虱不同生物型抗性的HPLC分析 |
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| 引用本文： | 赵颖,黄凤宽,童晓立,庞雄飞.水稻品种对褐飞虱不同生物型抗性的HPLC分析[J].华南农业大学学报,2005,26(2):52-55. |
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| 作者姓名： | 赵颖 黄凤宽 童晓立 庞雄飞 |
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| 作者单位： | 华南农业大学,理学院,广东,广州,510642;华南农业大学,昆虫学系,广东,广州,510642;广西农业科学院,植物保护研究所,广西,南宁,530007;华南农业大学,昆虫学系,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金重点项目 (39930 12 0 )，华南农业大学校长基金资助项目 (2 0 0 2 0 4 7) |
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| 摘 要： | 借助高效液相色谱检测了对褐飞虱2种生物型具有不同抗性水平的2类水稻品种中的13个次生化合物色谱峰的差异，并运用多元回归分析法，分别建立了水稻品种对褐飞虱2种生物型的抗性级别与这些次生化合物含量之间的回归模型．结果表明，被测的13个组分中，峰1、峰2、峰8、峰12是影响水稻对褐飞虱生物型Ⅱ抗性水平的主要抗原次生化合物；峰3、峰4、峰5、峰9、峰11、峰12则是水稻抗褐飞虱孟加拉型的主要抗原次生化合物．提出了以抗原次生物质为标记的快速分析、鉴定、预测水稻对褐飞虱生物型Ⅱ、孟加拉型抗性水平的新途径．
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| 关 键 词： | 水稻 褐飞虱 生物型 高效液相色谱(HPLC) |
| 文章编号： | 1001-411X(2005)02-0052-04 |
HPLC analysis of rice variety resistance to different biotypes of Nilaparvata lugens |
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| ZHAO Ying.HPLC analysis of rice variety resistance to different biotypes of Nilaparvata lugens[J].Journal of South China Agricultural University,2005,26(2):52-55. |
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| Authors: | ZHAO Ying |
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| Abstract: | The chromatographic peak distinction of 13 secondary compounds from two types of rice varieties were investigated by using high-performance liquid chromatography(HPLC). The result of multiple regression analysis was showed that peak 1, peak 2, peak 8 and peak 12 were major secondary compounds to affect the variety resistance to brown planthopper (BPH, Nilaparvata lugens) biotype II, whereas peak 3, peak 4, peak 5, peak 9, peak 11 and peak 12 were major secondary compounds to Bangladesh biotype of BPH. Two regression equations among the area values of the chromatographic peaks and the BPH-resistant level of rice variety to different biotypes were obtained. It was demonstrated that the resistant activity of rice varieties to different BPH biotypes was closely associated with qualitative and quantitative combinations of the secondary compounds. These secondary compounds were suggested to be used as the marks to evaluated and identified the rice variety resistance to different biotypes of BPH. |
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| Keywords: | rice Nilaparvata lugens biotype HPLC |
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