| 房山区4种动物源性成分PCR检测试验 |
| |
| 引用本文： | 田璐,王伟青,肖海峻,杨新建.房山区4种动物源性成分PCR检测试验[J].北京农业职业学院学报,2019,33(4):26-29. |
| |
| 作者姓名： | 田璐 王伟青 肖海峻 杨新建 |
| |
| 作者单位： | 北京农业职业学院,北京,102442;北京农业职业学院,北京,102442;北京农业职业学院,北京,102442;北京农业职业学院,北京,102442 |
| |
| 基金项目： | 北京农业职业学院2012年度院级科研项目(XY-BS-12-05) |
| |
| 摘 要： | 本研究利用牛、羊、猪、鸡线粒体mtDNA中保守序列16s rRNA，设计一对动物的通用引物，检测了含有动物源性成分的样品，同时设计了针对牛、羊、猪、鸡mtDNA中的保守序列，选择4对特异性引物，扩增得到目的片段大小分别为271 bp、274 bp、212 bp和266 bp。利用通用引物，优化了PCR反应体系及其反应条件，建立了从未知样品中快速获取动物源性成分检测的PCR方法；利用牛、羊、猪、鸡特异性引物，建立了PCR方法快速鉴定牛、羊、猪、鸡这4种动物源性成分的方法。
|
| 关 键 词： | 动物源性成分 动物食品 PCR |
| 收稿时间： | 2018/12/5 0:00:00 |
Research on the PCR Test of Four Species of Animal Derived Materials in Fangshan District, Beijing |
| |
| TIAN Lu,WANG Wei-qing,XIAO Hai-jun,YANG Xin-jian.Research on the PCR Test of Four Species of Animal Derived Materials in Fangshan District, Beijing[J].Journal of Beijing Agricultural Vocation College,2019,33(4):26-29. |
| |
| Authors: | TIAN Lu WANG Wei-qing XIAO Hai-jun YANG Xin-jian |
| |
| Institution: | Beijing Vocational College of Agriculture, Beijing 102442, China |
| |
| Abstract: | A general primer was designed according to 16S rRNA gene sequence of cattle, sheep,
pigs and chicken mtDNA in order to detect animal derived materials in foodstuff. Three species-specific
primers were designed according to conserved sequence of mtDNA of cattle, sheep, pigs, and chicken
were used for amplification of 271, 274 , 212 and 266 bp fragments respectively. PCR detection of animal
derived materials in unknown foodstuff was developed by using general primer and optimizing relevant
system and PCR condition. Also PCR detection method of cattle, sheep, pigs and chicken was designed
by applying species-specific primers. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《北京农业职业学院学报》浏览原始摘要信息 |
| 点击此处可从《北京农业职业学院学报》下载免费的PDF全文 |
|
