| 2017年福建地区PRRSV分子检测及ORF5基因变异分析 |
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| 引用本文： | 韦应芳,冀池海,孙彦阔,韩晓亮,陈尧,张桂红.2017年福建地区PRRSV分子检测及ORF5基因变异分析[J].华南农业大学学报,2019,40(3):14-19. |
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| 作者姓名： | 韦应芳 冀池海 孙彦阔 韩晓亮 陈尧 张桂红 |
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| 作者单位： | 国家生猪种业工程技术研究中心/广东省动物源性人兽共患病预防与控制重点实验室/华南农业大学兽医学院,广东广州,510642;江苏恒丰强生物技术有限公司,江苏南通,226100 |
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| 基金项目： | 国家重点研发计划项目（2018YFD0501200）；国家生猪现代农业产业技术体系项目（CARS-35）；国家自然科学基金（31272564） |
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| 摘 要： | 目的 研究2017年福建地区中小规模猪场猪繁殖与呼吸综合征病毒（PRRSV）毒株的分子流行情况。方法 从福建地区中小规模猪场收集83份疑似蓝耳阳性病料，对编码GP5蛋白的ORF5基因进行了RT-PCR检测和遗传进化分析。结果 采集的83份疑似蓝耳病料中阳性率为73%，测得的序列之间ORF5的核苷酸相似性达80.3%~100.0%，其中所测的各个分支毒株与其所属分支的代表毒株NADC30、GM2和JXA1的核苷酸相似性分别为92.6%~93.9%、89.8%~93.0%和93.4%~99.3%，检测到新分支中新出现的毒株与JXA1的核苷酸相似性为91.0~93.0%。GP5氨基酸比对结果显示不同亚群GP5信号肽区、诱导表位、主要中和表位及高变区等功能区发生了显著变异。结论 福建地区出现了PRRSV新分支，使福建PRRSV变异种类更加多样化，建议加强PRRSV流行及变异的监测。
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| 关 键 词： | 猪繁殖与呼吸道综合征 蓝耳病 分子流行病学 ORF5基因 遗传变异 |
| 收稿时间： | 2018/10/18 0:00:00 |
Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017 |
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| WEI Yingfang,JI Chihai,SUN Yankuo,HAN Xiaoliang,CHEN Yao and ZHANG Guihong.Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017[J].Journal of South China Agricultural University,2019,40(3):14-19. |
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| Authors: | WEI Yingfang JI Chihai SUN Yankuo HAN Xiaoliang CHEN Yao and ZHANG Guihong |
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| Institution: | National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Jiangsu Hengfengqiang Biotechnology Co., Ltd., Nantong 226100, China and National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Objective To study the molecular prevalence of the porcine peproductive and respiratory syndrome virus (PRRSV) strain in small and medium-sized pig farms of Fujian province in 2017.Method We collected 83 suspected blue ear positive disease materials from small and medium-sized pig farms in Fujian province. RT-PCR detection and genetic evolution analysis of ORF5 gene which encoded GP5 protein was performed.Result The positive rate of 83 suspected blue ear disease samples was 73%, and the nucleotide similarity among the measured ORF5 sequences was 80.3%-100.0%. The nucleotide similarities between tested strains of different branches and the representative strains of those branches, NADC30, GM2 and JXA1, were 92.6%-93.9%, 89.8%-93.0% and 93.4%-99.3%, respectively. The new identified strain in the new branch had nucleotide similarity of 91.0%-93.0% to JXA1. The GP5 amino acid alignment showed significant variation in functional regions such as GP5 signal peptide region, induction epitope, principal neutralizing epitope and hypervariable region in different subgroups.Conclusion A new branch of PRRSV has emerged in Fujian area, making the PRRSV variants in Fujian more diverse. It is necessary to strengthen the monitoring of PRRSV epidemics and mutations. |
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| Keywords: | porcine reproductive and respiratory syndrome blue ear disease molecular epidemiology ORF5 gene genetic variation |
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