| 6个枣品种枣疯病抗性与枣疯植原体迁移特性差异研究 |
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| 引用本文： | 郝新迪,路瑾瑾,代丽珍,张铁强,王进忠,王龙,郭家洛,任争光,王建文.6个枣品种枣疯病抗性与枣疯植原体迁移特性差异研究[J].果树学报,2021(4):560-568. |
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| 作者姓名： | 郝新迪 路瑾瑾 代丽珍 张铁强 王进忠 王龙 郭家洛 任争光 王建文 |
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| 作者单位： | 北京农学院植物科学技术学院;北京农学院生物与资源环境学院 |
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| 基金项目： | 北京市自然科学基金与北京市教育委员会科技计划重点项目(KZ201810020026);北京市自然科学基金(6182002);北京农学院枣树资源圃建设(5026016090)。 |
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| 摘 要： | 【目的】探究6个枣树品种枣疯病抗性差异以及枣树体内植原体迁移特性差异,为抗枣疯病枣树品种的选择利用以及枣疯病的防治提供基础数据。【方法】通过采集携带植原体的鸡心脆枣接穗,嫁接到健康枣树砧木上,采用田间调查与普通PCR技术相结合方法,比较不同品种抗枣疯病情况与植原体在不同枣树品种中迁移情况。【结果】不同枣树品种发病时间不同,发病率差异显著,蜂蜜罐枣、尜尜枣和猴头枣为抗病类型,冬枣、红螺脆枣和鸡心脆枣为感病类型。植原体在不同枣树品种中迁移速度不同,植原体在鸡心脆枣和冬枣内迁移速度最快,其后依次为蜂蜜罐枣、红螺脆枣、尜尜枣,在猴头枣内迁移速度最慢。【结论】6个枣树品种中蜂蜜罐枣、尜尜枣和猴头枣表现一定的抗病能力,初步确认了植原体在不同枣树品种体内迁移特征的差异,可为抗枣疯病品种的选择利用和枣疯病防治提供科学依据。
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| 关 键 词： | 枣疯病 抗病性 嫁接传病 植原体 迁移 |
Resistance of six varieties to witches-broom disease and difference in migration characteristics of phytoplasma in jujube |
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| HAO Xindi,LU Jinjin,DAI Lizhen,ZHANG Tieqiang,WANG Jinzhong,WANG Long,GUO Jialuo,REN Zhengguang,WANG Jianwen.Resistance of six varieties to witches-broom disease and difference in migration characteristics of phytoplasma in jujube[J].Journal of Fruit Science,2021(4):560-568. |
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| Authors: | HAO Xindi LU Jinjin DAI Lizhen ZHANG Tieqiang WANG Jinzhong WANG Long GUO Jialuo REN Zhengguang WANG Jianwen |
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| Institution: | (College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;School of Biology,Resources and Environment,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | 【Objective】Jujube is a unique fruit crop in China with a long cultivation history.However,jujube witches-broom disease caused by phytoplasma seriously threatens the yield of jujube trees.Therefore,in this study,six main jujube varieties in Beijing area including Gagazao,Dongzao,Fengmiguan,Jixincuizao,Hongluocuizao and Houtouzao served as the test materials.This study aimed to explore the differences in resistance to jujube witches-broom disease and the phytoplasma migrating characteristics in six varieties,which would provide reliable basic reference for the development and utilization of resistant varieties and the prevention and treatment of jujube witches-broom disease.【Methods】By collecting Jixincuizao with phytoplasma and grafting it onto the healthy jujube rootstock,the disease incidence rate was investigated every 30 days from July to October 2016,and from 2017 to 2018.Phloem and leaves of 5 cm above the graft union,including the first,second and third branches,and 5 cm,10 cm,15 cm and 20 cm below the graft union were collected after they were grafted 39 d,60 d,90 d,120 d and 300 d.In the summer of 2017 and spring of 2018,we collected leaves and phloem at 5 cm above the graft union and 20 cm below the graft union.The combination of field investigation and common PCR technology were used to compare the resistance of 6 varieties to jujube witches-broom disease and migration features of phytoplasma to different parts.【Results】The results showed that,60 d after grafting,none of the 6 varieties of seedlings showed symptoms of jujube witches-broom disease;90 d after grafting,Dongzao,Fengmiguanzao and Jixincuizao exhibited symptoms,and the incidence rates of the three varieties were:13.8%(Jixincuizao),11.6%(Dongzao),and 4.1%(Fengmiguanzao).However,120 d after grafting except for Houtouzao,the other 5 varieties all showed symptoms,and the incidence rates were:32.5%(Jixincuizao),18.4%(Hongluocuizao),29.0%(Dongzao),8.4%(Gagazao)and 23.3%(Fengmiguanzao,respectively.After two years of grafting,all 6 varieties had symptoms of jujube witches-broom disease,and the incidence rates were:79.3%(Jixincuizao),69.6%(Hongluocuizao),67.7%(Dongzao),57.6%(Gagazao),51.2%(Fengmiguanzao),and 49.0%(Houtouzao),respectively,indicating that none of the 6 jujube varieties was immune to phytoplasma,and different jujube varieties were differentially resistant to the disease and the difference was significant.According to the onset time and incidence rate,cultivars such as Houtouzao,Fengmiguanzao and Gagazao belonged to disease-resistant type,and Dongzao,Hongluocuizao and Jixincuizao belonged to susceptible type.After grafting with phytoplasma branches,the phytoplasma was first transferred to the trunk below the rootstock grafting interface,and then transferred to the trunk above the grafting interface,and finally spread to the whole plant branches.In addition,the migration speed of phytoplasma was different among different jujube varieties.Phytoplasma migrated fastest in Jixincuizao and Dongzao,followed by Fengmiguanzao,Hongluocuizao,and Gagazao,and the migration speed was the slowest in Houtouzao.【Conclusion】Among six varieties,Fengmiguanzao,Gagazao and Houtouzao showed a certain disease resistance,and the migration characteristics of phytoplasma in different jujube varieties were preliminarily confirmed,whose phytoplasma migrated fastest in Jixincuizao and Dongzao,followed by Fengmiguanzao,Hongluocuizao,and Gagazao,and the migration speed was the slowest in Houtouzao.However,due to the limitation of the sample number of jujube varieties,whether the migration speed of phytoplasma was positively correlated with the resistance of different jujube species needed deep research further. |
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| Keywords: | Jujube witches-broom disease Resistance Graft transmission Phytoplasma detection Migration |
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