| 朝鲜蓟叶高效液相色谱指纹图谱的初步研究 |
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| 引用本文： | 宋曙辉,霍超,王文琪,何洪巨,张宝海.朝鲜蓟叶高效液相色谱指纹图谱的初步研究[J].安徽农业科学,2010,38(20):10655-10658,10701. |
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| 作者姓名： | 宋曙辉 霍超 王文琪 何洪巨 张宝海 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心,北京,100097;北京市农林科学院蔬菜研究中心,北京,100097;北京市农林科学院蔬菜研究中心,北京,100097;北京市农林科学院蔬菜研究中心,北京,100097;北京市农林科学院蔬菜研究中心,北京,100097 |
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| 基金项目： | 北京市科技新星计划项目 |
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| 摘 要： | 目的]采用高效液相色谱法建立朝鲜蓟叶提取物的指纹图谱。方法]以体积分数为70%的乙醇作为提取剂,采用C18色谱柱（4.6mm×250.0mm,5μm）,乙腈-0.5%乙酸-水为流动相梯度洗脱,检测波长为330nm。结果]14批样品经中药指纹图谱评价软件分析,确定了15个共有峰,建立了共有模式。结论]该方法建立的指纹图谱为朝鲜蓟叶深加工的质量控制奠定了基础。
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| 关 键 词： | 朝鲜蓟叶 高效液相色谱 指纹图谱 相似度 共有模式 |
Preliminary Study on HPLC Fingerprint of Artichoke (Cynara scolymus L.) Leaves |
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| Institution: | SONG Shu-hui et al(Beijing Vegetable Research Center,BAAFS,Beijing 100097) |
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| Abstract: | Objective]To establish the HPLC fingerprint of artichoke(Cynara scolymus L.)leaves extracts.Method]The artichoke leaves was extracted with 70% ethanol(V/V)and analyzed with HPLC,and the chromatographic condition were as follows:C18 column(4.6 mm×250.0 mm,5 μm),mobile phases of acetonitrile-0.5% acetic acid and detection wavelength of 330 nm.Result]Fourteen bacthes of the extracts were analyzed by Chinese Medicine Chromatographic Fingerprint Similarity Evaluation software,and 15 peaks were identified as fingerprint.The common mode of artichoke leaves extracts was preliminary established.Conclusion]The fingerprint established by this method will provide basic data for the quality control of artichoke leaves. |
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| Keywords: | Cynara scolymus L leaves HPLC Fingerprint Similarity Common mode |
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