| 一株副球菌对污染土壤中多环芳烃的降解研究 |
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| 引用本文： | 毛 健,骆永明,滕 应,李振高.一株副球菌对污染土壤中多环芳烃的降解研究[J].土壤,2009,41(3):448-453. |
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| 作者姓名： | 毛 健 骆永明 滕 应 李振高 |
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| 作者单位： | 1. 中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),南京,210008;中国科学院研究生院,北京,100049
2. 中国科学院土壤环境与污染修复重点实验室(中国科学院南京土壤研究所),南京,210008 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目，国家重点基础研究发展规划(973计划)，国家高技术研究发展计划(863计划) |
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| 摘 要： | 从受多环芳烃(PAHs)长期污染的土壤中分离到一株降解PAHs的噬氨副球菌(Paracoccus aminovorans)HPD-2.使用HPD-2的菌液对PAHs污染的土壤进行了2周的生物降解试验,结果表明加入HPD-2能够明显提高土壤中PAHs的降解率.加菌土壤中PAHs的总去除率为22.9%,PAHs各组分的降解率在19.5% ～ 36.2% 之间.其中三环PAHs的降解率最高(36.1%),五环次之(26.0%),四环的最低(20.9%).对土壤微生物的计数结果发现,HPD-2的加入显著提高了土壤中细菌的数量,而对放线菌和真菌的影响不明显.PCR-DGGE分析结果表明,降解过程中HPD-2可能成为土壤中的优势菌.以上结果表明该菌株在PAHs污染土壤的生物降解中具有较好的应用前景.
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| 关 键 词： | 噬氨副球菌 多环芳烃 生物降解 土壤 |
Biodegradation of PAHs by Paracoccus aminovorans HPD-2 in Contaminated Soil |
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| MAO Jian,LUO Yong-ming,TENG Ying,LI Zhen-gao.Biodegradation of PAHs by Paracoccus aminovorans HPD-2 in Contaminated Soil[J].Soils,2009,41(3):448-453. |
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| Authors: | MAO Jian LUO Yong-ming TENG Ying LI Zhen-gao |
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| Institution: | Key Laboratory of Soil Environment and Pollution Remediation, Chinese Academy of Sciences, (Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China |
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| Abstract: | The effects of the inoculant strain Paracoccus aminovorans HPD-2 (isolated from a PAH-contaminated soil) on the bioremediation of the PAHs-contaminated soil and the structure of microbial communities in the soil were studied in soil microcosms. After inoculation for 14 days, 22.9% of total PAHs were removed from the soil in the treatment with the addition of 20% (v/m) bacterium suspension. The removal of 3-rings PAHs (36.1%) was higher than that of 5-rings (26.0%) and 4-rings PAHs (20.9%). The highest degradation of PAHs occurred in phenanthrene, anthracene, and benzoa]pyrene. Bacterial population in the soil increased during the bioremediation process, but the population of actinomycetes and fungi changed insignificantly. Denaturing gradient gel electrophoresis (DGGE) analysis of polymerase chain reaction (PCR)-amplified bacterial 16S rRNA gene fragments revealed that HPD-2 became into the dominant bacterial group in the soil with the addition of the bacterium suspension. These results indicate that HPD-2 is a promising candidate for bioremediation of PAHs-contaminated soils. |
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| Keywords: | PCR-DGGE |
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