| 长蕊万寿竹野生资源开发利用的组织培养研究 |
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| 引用本文： | 蒋天仪,石大兴,王波.长蕊万寿竹野生资源开发利用的组织培养研究[J].安徽农业科学,2008,36(31). |
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| 作者姓名： | 蒋天仪 石大兴 王波 |
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| 作者单位： | 成都农业科技职业学院农学园艺系,四川成都,611130;四川农业大学林学系,四川雅安,625014;四川工程职业技术学院建筑工程系,四川德阳,618000 |
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| 摘 要： | 目的]为长蕊万寿竹的大规模生产和进一步研究提供技术支持和参考依据。方法]以长蕊万寿竹茎段为外植体,灭菌后将带芽茎段剪成1~2 cm的小段,接种于培养基中。研究外源激素的种类与配比对诱导生根和丛生芽的影响。结果]启动培养时,外源激素为6-BA 1.0 mg/L+2,4-D 0.01 mg/L+NAA 0.1 mg/L+IBA 1.0 mg/L的培养基的效果最好,启动率达到85.20%。1/2 MS添加外源激素NAA 1.0 mg/L+AC 5 g/L的培养基的生根效果极显著地高于其它处理,生根率达到96.13%。适合丛生芽诱导的培养基是6-BA 0.5mg/L+KT 1.5 mg/L+NAA 0.2 mg/L+蔗糖30 g/L,丛芽增殖系数达到6.1。结论]建立了以长蕊万寿竹茎段为外植体的组织培养技术体系,可有效地启动培养,诱导生根和丛生芽。
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| 关 键 词： | 长蕊万寿竹 野生资源 组织培养 |
Study on Tissue Culture of Disporum bodinieri (Levl. et.Vnt.) Wang et Tang |
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| Abstract: | Objective] The study aimed to provide the technical support and reference for large scale production and further research of Disporum bodinieri (Levl.et.Vnt.) Wang et Tang.Method] With the stem of D.bodinieri seedlings as explants,the sterilized stem with bud was cut into 1-2 cm small part and then inoculated into the medium.The effects of type and ratio of exogenous hormone on rooting and cluster bud induction were studied.Result] When the culture was initiated,the effect of medium with exogenous hormone of 6-BA 1.0 mg/L + 2,4-D 0.01 mg/L+ NAA 0.1 mg/L+ IBA 1.0 mg/L was the best,the initiation rate reached 85.20%.The rooting effect of 1/2 MS medium with the exogenous hormones of NAA 1.0 mg/L+AC 5 g/L was extremely higher than that of other treatments and the rooting rate reached 96.13%.The medium suitable for cluster bud induction was 6-BA 0.5 mg/L+KT 1.5 mg/L +NAA 0.2 mg/L + sucrose 30 g/L,and the multiplication coefficient of cluster bud reached 6.1.Conclusion] The technique system of tissue culture with the stem of D.bodinieri seedlings as explants was established,with which the culture could be initiated and the rooting and cluster bud could be induced effectively. |
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| Keywords: | Disporum bodinieri(Levl et Vnt ) Wang et Tang Wild resources Tissue culture |
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