| 高效液相色谱法测定鱼粉中7种生物胺含量 |
| |
| 引用本文： | 杨丽云,刘成模,肖锦永,黄智成,李娟.高效液相色谱法测定鱼粉中7种生物胺含量[J].中国畜牧兽医,2013,40(12):112-115. |
| |
| 作者姓名： | 杨丽云 刘成模 肖锦永 黄智成 李娟 |
| |
| 作者单位： | 1. 广东恒兴饲料实业股份有限公司, 广东湛江 524094;2. 广东省农业科学院动物卫生研究所, 广东广州 510640 |
| |
| 基金项目： | 广东省农业科技园区项目（2011A020504005）。 |
| |
| 摘 要： | 试验以0.4 mol/L高氯酸溶液为样品提取溶剂，以丹磺酰氯为衍生剂，以乙腈和0.02 mol/L乙酸铵溶液为流动相，采用梯度洗脱，于波长254 nm处紫外检测，同时建立检测鱼粉中7种生物胺的反相高效液相色谱法（RP-HPLC）。结果表明，在45 min内各种生物胺得到很好分离，经方法学验证线性关系良好，回收率在85%~105%之间，精密度相对标准偏差（RSD）均小于4%。所建立方法灵敏度高，重现性好，是测定鱼粉中生物胺含量的有效方法。
|
| 关 键 词： | 生物胺 鱼粉 高效液相色谱法 |
| 收稿时间： | 2013-06-04 |
Determination of Seven Kinds of Biogenic Amines in Fishmeal by a High Performance Liquid Chromatography Method |
| |
| YANG Li-yun,LIU Cheng-mo,XIAO Jin-yong,HUANG Zhi-cheng,LI Juan.Determination of Seven Kinds of Biogenic Amines in Fishmeal by a High Performance Liquid Chromatography Method[J].China Animal Husbandry & Veterinary Medicine,2013,40(12):112-115. |
| |
| Authors: | YANG Li-yun LIU Cheng-mo XIAO Jin-yong HUANG Zhi-cheng LI Juan |
| |
| Institution: | 1. Guangdong Evergreen Feed Industry Co., Ltd., Zhanjiang 524094, China;2. Institute for Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China |
| |
| Abstract: | Biogenic amines were extracted by 0.4 mol/L HCLO4 solution. Derivatisation of the samples was carried out using dansyl chloride. A gradient elution system was used for analyzing a mixture of acetonitrile and 0.02 mol/L ammonium acetate solutions as well as UV detector were applied at 254 nm to measure 7 kinds of biogenic amines simultaneously in fishmeal. The established method had a good resolution in 45 minutes and showed good linearity. Its recovery rate belonged to 85% to 105%,RSD of precision were all lower than 4%. It showed that the established method was feasible for the determination of biogenic amines in fishmeal. |
| |
| Keywords: | biogenic amines fishmeal high performance liquid chromatography method |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
