Establishment of Real-time PCR Assays for Detection of the Intramuscular Fat Deposition Related Genes in Mongolia Sheep |
| |
| Authors: | MIAO Hai-ming GU Yue YANG Jin-li GAO Ai-wu HUANG Ya-juan WANG Hai-rong |
| |
| Institution: | 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China |
| |
| Abstract: | This experiment was conducted to establish a detection method which was suitable for genes expression of Mongolia sheep intramuscular fat (IMF) deposition genes. According to the sequences of IMF deposition genes in GenBank, pairs of specific primers were designed and synthesized. Then the amplified fragments were detected by cloning technology to ensure the accuracy of the PCR products. The Real-time fluorescent quantitative PCR (Real-time PCR) was used to check the feasibility of method. Results showed that Real-time PCR optimum cycle parameters were 94 ℃ 4 min, 94 ℃ 30 s, 58 ℃ 45 s, 72 ℃ 1 min,40 cycles;72 ℃ 5 min.Each peak melting curve was a single gene, the target product was stable. The method of Real-time PCR was established successfully and could be specially used to detect IMF deposition genes of Mongolia sheep. |
| |
| Keywords: | Mongolia sheep fat deposition Real-time PCR establishment method |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
