| 苏尼特羊拷贝数变异的基因组分布特征研究 |
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| 引用本文： | 刘佳森,张莉,李蕴华,赵福平,魏彩虹,杜立新.苏尼特羊拷贝数变异的基因组分布特征研究[J].中国畜牧兽医,2013,40(10):173-178. |
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| 作者姓名： | 刘佳森 张莉 李蕴华 赵福平 魏彩虹 杜立新 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;2. 内蒙古农牧业科学院畜牧研究所, 内蒙古呼和浩特 010031 |
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| 基金项目： | 国家自然科学基金(30972094);十二五863项目(2011AA100307-02);国家现代农业产业－肉羊体系(CARS-39-04B);内蒙古农牧业科学院青年创新基金(2011QNJJM07)。 |
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| 摘 要： | 本试验利用Illumina OvineSNP50 BeadChip芯片对71只苏尼特羊进行了分型,共检测到134个拷贝数变异区域(copy number variation regions,CNVR),大小范围为29.48 kb~1.30 Mb之间,总长度达到25.95 Mb。基因注释及功能分析结果显示,这些基因与嗅觉感官知觉、化学刺激的感官知觉、感官知觉、识别等环境应答有关。选取5个CNVR进行qPCR验证,其中3个CNVR得到验证。通过对苏尼特羊基因组拷贝数变异的分析可以进一步了解绵羊基因组结构的特点,为今后开展绵羊基因组结构变异与重要经济性状的关联研究提供参考。
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| 关 键 词： | 苏尼特羊 基因组 拷贝数变异 单核苷酸多态 |
| 收稿时间： | 2013-03-18 |
Genomic Characteristics of Copy Number Variations in Sunite Sheep |
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| LIU Jia-sen,ZHANG Li,LI Yun-hua,ZHAO Fu-ping,WEI Cai-hong,DU Li-xin.Genomic Characteristics of Copy Number Variations in Sunite Sheep[J].China Animal Husbandry & Veterinary Medicine,2013,40(10):173-178. |
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| Authors: | LIU Jia-sen ZHANG Li LI Yun-hua ZHAO Fu-ping WEI Cai-hong DU Li-xin |
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| Institution: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Institute of Animal Science, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia Autonomous Region, Hohhot 010031, China |
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| Abstract: | In this study, 71 Sunite sheep were genotyped using Illumina OvineSNP50 BeadChip array, a total of 134 CNV regions (CNVR) across the genome were identified, which covered 25.95 Mb of the sheep genome.CNVR size on autosomes range from 29.48 kb to 1.30 Mb. Functional indicated that the most genes overrepresented in environmental responses, which involved in sensory perception of smell, sensory perception of chemical stimulus, sensory perception, cognition. To confirm these findings, quantitative PCR (qPCR) was performed for 5 CNVR and 3 of them were successfully validated. Our findings would be of help to understand the sheep genome and provide preliminary foundation for investigating the association between important economic traits and CNV. |
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| Keywords: | Sunite sheep genome copy number variation single nucleotide polymorphism |
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