Preliminary Study on Metabolic Resistance Mechanism of Aphis craccivora in Intercropping Field of Cotton and Peanut to Imidacloprid |
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| Authors: | Yang Yuanxue Li Zhuo Duan Ailing Xue Chao Xu Zhiping Zhao Ming |
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| Institution: | 1. Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2. School of Pharmacy, EastChina University of Science and Technology, Shanghai 200237, China |
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| Abstract: | Objective] This study aims to explore the difference of imidacloprid resistance between Aphid gossypii and Aphid craccivora in intercropping field of cotton and peanut, and the mechanism of imidacloprid resistance to A. craccivora, so as to scientifically control these pests and to effectively avoid the rapid development of the resistance to imidacloprid. Method] Two A. gossypii field populations and two A. craccivora field populations were collected from intercropping field of cotton and peanut in Juye and Linqing county of Shandong province, China. The bioassay experiment with two A. gossypii field populations, the bioassay and synergism experiment in two A. craccivora field populations were performed by the leaf dipping method. In addition, the activities of carboxylesterase (CarE), glutathione S-transferase (GST) and cytochrome P450 monooxygenase (P450) were assayed in the susceptible strain and two field populations of A. craccivora. Result] Two A. gossypii field populations from Linqing and Juye exhibited moderate levels of resistance to imidacloprid, with the resistance ratios of 43.2- and 54.6-fold, while two A. craccivora field populations from Linqing and Juye showed susceptible and low level of resistance to imidacloprid, with the resistance ratio of 3.7- and 8.3-fold, respectively. According to the synergistic experiments, PBO and DEM significantly synergized imidacloprid in A. gossypii field population of Juye with the synergistic ratio of 3.63- and 1.95-fold, respectively, and TPP had no effect on imidacloprid toxicity. In A. craccivora field population of Linqing, PBO significantly synergized imidacloprid with the synergistic ratio of 3.05-fold, and DEM and TPP had no effect on imidacloprid. Further enzyme activity tests revealed that the activities of P450 and GST in Juye A. craccivora population were significantly higher than susceptible strain, and the activity of CarE had no significant difference between Juye A. craccivora population and susceptible strain. However, the activity of P450 in Linqing A. craccivora population was higher than susceptible strain, and the activities of CarE and GST had no significant differences. Conclusion] Sensitivity of the two aphids in intercropping field of cotton and peanut to imidacloprid were greatly different, and P450 and GST may play an important role in resistance of A. craccivora to imidacloprid. The results are valuable for reasonable use of pesticides to delay the development of pesticide resistance of two aphids. |
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| Keywords: | Aphid gossypii Aphis craccivora intercropping field imidacloprid metabolic resistance |
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