Identification of the microRNAome in Texel Sheep by Deep Sequencing |
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| Authors: | ZHANG Shi-fang WEI Cai-hong LU Jian ZHANG Xiao-ning ZHOU Xin-lei ZHANG Shu-zhen WANG Guang-kai CAO Jia-xue ZHAO Fu-ping ZHANG Li DU Li-xin |
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| Institution: | National Center for Molecular Genetics and Breeding of Animal, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Information concerning the sheep microRNA (miRNA) had been long overdue, the discovery and identification of miRNAs would further promote the study of miRNA functions and gene regulatory mechanisms. Samples were culled that included five developmental stages: The longissimus dorsi muscles of Texel fetuses were at 70, 85, 100, 120 and 135 days. We performed a comprehensive search for Texel miRNA on the pool library by Solexa deep sequencing; then obtained 16532850 raw reads. This study used the ACGT101-miR v4.2 analysis software to explore the sequencing data. The sequencing results were analyzed using the latest mammalian mature miRNA (in miRBase v17.0), precursors (pre-miRNAs), and sheep genome (Ovis aries genome database, Feb 2010). Our results extend the repertoire of sheep microRNAome to 1529 pre-miRNAs encoding for 1999 mature miRNA. We preformed quantitative Real-time PCR experiments for selected 5 miRNA in the pool sample and found agreement between the sequencing and Real-time PCR data. These results were prelude to the advancement in sheep biology. |
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| Keywords: | Solexa sequencing Texel sheep longissimus dorsi muscle microRNAome |
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