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| 猪输卵管特异性糖蛋白结构与功能的生物信息学分析 | |
| 引用本文： | 刘春英,李方正.猪输卵管特异性糖蛋白结构与功能的生物信息学分析[J].中国畜牧兽医,2013,40(8):20-25. |
| 作者姓名： | 刘春英 李方正 |
| 作者单位： | 1. 青岛农业大学生命科学学院, 山东青岛 266109; 2. 青岛农业大学动物科技学院, 山东青岛 266109 |
| 基金项目： | 青岛农业大学高层次人才科研基金资助(631209)。 |
| 摘 要： | 利用Lasergene、Protscale、SOPMA、Mega、ScanProsite等生物信息学软件对猪输卵管特异性糖蛋白(oviduct-specific glycoprotein,OGP)进行物理性质、结构及系统进化分析。结果表明,猪OGP cDNA包含1个1581 bp的开放阅读框,编码527个氨基酸,有信号肽,属于分泌蛋白,无明显疏水区;猪OGP蛋白二级结构主要是α-螺旋和不规则卷曲。系统进化分析结果表明,猪OGP蛋白与绵羊OGP亲缘关系最近,与牛OGP的亲缘关系较远。提示,OGP蛋白属于糖苷水解酶18(glycosyl hydrdase 18 family,GH18)家族,牛OGP的GH18结构与猪的差别较大。 |
| 关 键 词： | 猪 输卵管特异性糖蛋白 生物信息学 |
| 收稿时间： | 2013-03-29 |
Bioinformatics Analysis of Porcine Oviduct-specific Glycoprotein Structure and Function |
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| LIU Chun-ying,LI Fang-zheng.Bioinformatics Analysis of Porcine Oviduct-specific Glycoprotein Structure and Function[J].China Animal Husbandry & Veterinary Medicine,2013,40(8):20-25. | |
| Authors: | LIU Chun-ying LI Fang-zheng |
| Institution: | 1. Life Science Department, Qingdao Agricultural University, Qingdao 266109, China; 2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China |
| Abstract: | Lasergene,Protscale,SOPMA,Mega and ScanProsite were used to study the structure, conservation and phylogeny of porcine oviduct-specific glycoprotein (OGP). The results showed that the full length of porcine OGP contained a complete open read coding of 1581 bp, which encoded 527 amino acids. The porcine OGP had obvious signal peptide and it was a secretion protein, it was no obvious hydrophobic region. The secondary structures were mainly composed of α-helix and random coli. Phylogenetic tree analysis of the amino acids sequences showed that the porcine OGP displayed nearest relationship with the sheep OGP, and lower homology with those of bovine. The OGP was a member of glycosyl hydrolase 18 family (GH18). The GH18 domain structure of OGP had high differences between bovine and porcine. |
| Keywords: | porcine oviduct-specific glycoprotein (OGP) bioinformatics |
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