| 酵母双杂交技术及应用的研究进展 |
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| 引用本文： | 郑立双,李向楠,孙城涛,刘红羽,刘勋,贺明,吕文发.酵母双杂交技术及应用的研究进展[J].中国畜牧兽医,2013,40(9):105-108. |
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| 作者姓名： | 郑立双 李向楠 孙城涛 刘红羽 刘勋 贺明 吕文发 |
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| 作者单位： | 1. 吉林农业大学生命科学学院, 吉林长春 130118;2. 吉林农业大学动物科学技术学院, 吉林长春 130118 |
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| 基金项目： | 国家自然科学基金(30972108);吉林省政府人才开发基金。 |
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| 摘 要： | 酵母双杂交技术是研究蛋白质与蛋白质相互作用的一种非常有效的分子生物学方法,它是一种能直接检测胞内蛋白质相互作用较为简便、快捷、高效、敏感、广泛的方法。作者将主要介绍酵母双杂交技术的基本原理、应用及优缺点,现就酵母双杂交技术及其应用的研究进展作一综述。
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| 关 键 词： | 酵母双杂交技术 真核细胞 蛋白质 |
| 收稿时间： | 2013-01-28 |
Research Progress on Yeast Two-hybrid Technology and Application |
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| ZHENG Li-shuang,LI Xiang-nan,SUN Cheng-tao,LIU Hong-yu,LIU Xun,HE Ming,LV Wen-fa.Research Progress on Yeast Two-hybrid Technology and Application[J].China Animal Husbandry & Veterinary Medicine,2013,40(9):105-108. |
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| Authors: | ZHENG Li-shuang LI Xiang-nan SUN Cheng-tao LIU Hong-yu LIU Xun HE Ming LV Wen-fa |
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| Institution: | 1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Yeast two-hybrid technology is an effective molecular biology method which is used to study the interaction of proteins. It is relatively simple, fast, efficient, sensitive, extensive and can directly detect the interaction of intracellular proteins. This paper mainly introduces the basic principle, applications, advantages and disadvantages of yeast two-hybrid technology, now the research on yeast two-hybrid system technology and application will be reviewed. |
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| Keywords: | yeast two-hybrid system eukaryotic cell protein |
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