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猪肉和牛奶源葡萄球菌耐药性调查
引用本文:魏鸿堃,冯言言,刘健华,吕世明,曾振灵.猪肉和牛奶源葡萄球菌耐药性调查[J].中国畜牧兽医,2013,40(10):203-206.
作者姓名:魏鸿堃  冯言言  刘健华  吕世明  曾振灵
作者单位:1. 华南农业大学兽医学院, 广东广州 510642;2. 贵州大学动物科学学院, 贵州贵阳 550025
基金项目:高等学校博士学科点专项科研(20104404110008)。
摘    要:2010年从贵州省猪肉和牛奶样品中分离鉴定出73株葡萄球菌,其中金黄色葡萄球菌50株,凝固酶阴性葡萄球菌23株。采用琼脂稀释法检测其对18种抗菌药物的敏感性,运用统计学方法比较分析金黄色葡萄球菌与凝固酶阴性葡萄球菌的耐药性差异。结果显示,动物性食品源葡萄球菌耐药较严重,对临床上常用药物耐药率较高,且为多重耐药,其中乳源葡萄球菌的多重耐药现象较猪肉源葡萄球菌严重;猪肉源金黄色葡萄球菌主要对青霉素类、四环素和大环内酯类耐药,乳源金黄色葡萄球菌主要对青霉素类、头孢菌素类、大环内酯类、四环素、克林霉素、泰妙菌素及利福平耐药;猪肉源凝固酶阴性葡萄球菌主要对氨苄西林、苯唑西林和泰妙菌素耐药,乳源凝固酶阴性葡萄球菌的耐药谱与乳源金黄色葡萄球菌类似。

关 键 词:金黄色葡萄球菌  凝固酶阴性葡萄球菌  耐药性  猪肉  牛奶  
收稿时间:2013-03-21

Survey on Antimicrobial Susceptibility of Staphylococcus Isolated from Pork and Milk
WEI Hong-kun,FENG Yan-yan,LIU Jian-hua,LV Shi-ming,ZENG Zhen-ling.Survey on Antimicrobial Susceptibility of Staphylococcus Isolated from Pork and Milk[J].China Animal Husbandry & Veterinary Medicine,2013,40(10):203-206.
Authors:WEI Hong-kun  FENG Yan-yan  LIU Jian-hua  LV Shi-ming  ZENG Zhen-ling
Institution:1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract:73 Staphylococcus strains including 50 Staphylococcus aureus (S.aureus) and 23 coagulase-negative Staphylococci were isolated from pork and milk samples with collected from Guizhou province in 2010. Antimicrobial susceptibility testing was performed with agar dilution method according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Statistical methods were used to analyze the differences of antimicrobial susceptibility between S.aureus and coagulase-negative Staphylococci which isolated from different samples. The results showed that these isolates displayed a high degree of resistance to the common antimicrobials and showed multi-drug resistance. Moreover, isolates which isolated from the milk samples showed much higher multi-drug resistance than those isolated from the pork samples. S.aureus which isolated from the pork samples demonstrated primarily resistant to penicillins,tetracyclines and macrolides,while S.aureus which isolated from the milk samples showed primarily resistant to penicillins,cephalosporins, macrolides, tetracyclines, clindamycin, tiamulin and rifampicin. The coagulase-negative Staphylococci which isolated from the pork samples displayed primarily resistant to ampicillin,oxacillin and tiamulin, and the resistance profile of coagulase-negative Staphylococci from milk samples showed no remarkable difference from that of the S.aureus isolated from the milk samples.
Keywords:Staphylococcus aureus  coagulase-negative Staphylococci  resistance  pork  milk  
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