Effect of gambogic acid on proliferation and apoptosis of human diffuse large B-cell lymphoma cell line OCI-LY-1 |
| |
| Authors: | SHI Xian-ping LAN Xiao-ying CHEN Xin WANG Wen-wen WEN Chuang-yu HUANG Mei-jin LIU Huan-liang |
| |
| Institution: | 1.Institute of Immunology, Guangzhou Medical University, Guangzhou 510182, China;2.Department of Hematology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;3.Institute of Gastroenterology, 4The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China. |
| |
| Abstract: | AIM: To investigate the effects of gambogic acid (GA) on the proliferation and apoptosis of human diffuse large B-cell lymphoma cells. METHODS: Diffuse large B-cell lymphoma cell line OCI-LY-19 was treated with GA. The cell viability and proliferation were evaluated by MTS assay. The morphological changes of the cells were observed under inverted microscope and fluorescence microscope with PI staining. The cell apoptosis was detected by flow cytometry. The changes of proteasome-related proteins Ubs, Bax, HSP90 and IκB-α, the apoptosis-related proteins PARP, pro-caspase-3 and caspase-8, and the proliferation-associated molecules ERK and STAT5 were detected by Western blotting. RESULTS: The proliferation of OCI-LY-19 cells was inhibited and the apoptosis was induced by GA. The expression of Ubs, Bax and HSP90 was up-regulated by GA treatment in a dose-dependent manner. The PARP protein was cleaved. The levels of apoptosis-related proteins pro-caspase-3 and pro-caspase-8, and proliferation-associated molecules p-Akt, p-ERK and p-STAT5 were down-regulated by GA treatment in a dose- and time-dependent manner. CONCLUSION: Gambogic acid down-regulates the activity of proteasome, inhibits cell proliferation and promotes cell apoptosis of diffuse large B cell lymphoma. |
| |
| Keywords: | Diffuse large B-cell lymphoma Gambogic acid Proteasome Cell proliferation Apoptosis |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
