| 早春季节罗非鱼源嗜水气单胞菌的分离鉴定 |
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| 引用本文： | 左跃,易弋,夏杰,罗福广,杨军,李方方,伍时华.早春季节罗非鱼源嗜水气单胞菌的分离鉴定[J].中国畜牧兽医,2013,40(10):228-230. |
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| 作者姓名： | 左跃 易弋 夏杰 罗福广 杨军 李方方 伍时华 |
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| 作者单位： | 1. 广西工学院生物与化学工程系, 广西柳州 545006;2. 柳州市渔业技术推广站, 广西柳州 545006;3. 国家罗非鱼产业技术体系柳州综合试验站, 广西柳州 545006 |
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| 基金项目： | 广西科技重大专项项目(桂科攻1123006-3);现代农业产业技术体系专项资金项目(CARS-49)。 |
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| 摘 要： | 2012年早春季节广西柳州市某罗非鱼养殖场出现暴发性疾病,从患病罗非鱼的肝脏、肾脏和脾脏组织中分离到1株革兰氏阴性杆菌。通过生理生化试验和16S rDNA序列分析,鉴定该菌为嗜水气单胞菌。人工感染试验结果显示,分离菌株对罗非鱼有很强的毒力,该菌注射浓度为1×109 CFU时,可使健康罗非鱼在24 h内全部死亡;药物敏感试验结果显示,分离菌株仅对氨苄西林耐药,对氨曲南、氧氟沙星等15种抗生素敏感。本研究为早春季节罗非鱼疾病防控提供试验依据。
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| 关 键 词： | 罗非鱼 嗜水气单胞菌 强毒力 早春季节 |
| 收稿时间： | 2013-03-27 |
Isolation and Identification of Aeromonas hydrophila from Infected Tilapia in Early Spring |
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| ZUO Yue,YI Yi,XIA Jie,LUO Fu-guang,YANG Jun,LI Fang-fang,WU Shi-hua.Isolation and Identification of Aeromonas hydrophila from Infected Tilapia in Early Spring[J].China Animal Husbandry & Veterinary Medicine,2013,40(10):228-230. |
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| Authors: | ZUO Yue YI Yi XIA Jie LUO Fu-guang YANG Jun LI Fang-fang WU Shi-hua |
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| Institution: | 1. College of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China;2. Liuzhou Fishery Technical Extension Station, Liuzhou 545006, China;3. Liuzhou Comprehensive Experiment Station of the National Tilapia Industry Technology System, Liuzhou 545006, China |
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| Abstract: | In early spring 2012, a disease outbreak occurred at a tilapia farm in Liuzhou of Guangxi province. A gram-negative bacillus was isolated from liver,kidney and spleen of infected tilapia. The isolate was identified as Aeromonas hydrophila by biochemical assays and 16S rDNA sequencing. Healthy tilapia injected with 1×109 CFU of the isolate reached a mortality of 100% in just 24 h. Drug sensitivity experiment showed that the isolate was only resistant to ampicillin and sensitive to 15 types of antibiotics such as aztreonam,ofloxacin,etc. This study provided a basis for the prevention and control of tilapia diseases in early spring. |
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| Keywords: | tilapia Aeromonas hydrophila strong virulence early spring |
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