| 饲料及其原料中沙门氏菌的分离鉴定和耐药分析 |
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| 引用本文： | 佘容,刘耀敏,杜雪莉,颜其贵.饲料及其原料中沙门氏菌的分离鉴定和耐药分析[J].中国畜牧兽医,2013,40(12):204-207. |
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| 作者姓名： | 佘容 刘耀敏 杜雪莉 颜其贵 |
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| 作者单位： | 1. 通威股份有限公司检测中心, 四川成都 610041;2. 四川农业大学动物医学院, 四川雅安 625014;3. 四川农业大学, 动物疫病与人类健康四川省重点实验室, 四川雅安 625014 |
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| 基金项目： | 教育部长江学者和创新团队发展计划项目（IRTO848）。 |
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| 摘 要： | 为了解饲料及其原料中沙门氏菌污染情况及耐药情况，为沙门氏菌病的防控工作提供理论依据，特针对饲料及其原料开展沙门氏菌污染情况及耐药情况筛查。试验共收集饲料及原料样品707份，参照GB/T 13091－2002饲料中沙门氏菌检测方法进行沙门氏菌的分离和鉴定；分离株以API 2.0E鉴定试剂盒确认；后参照GB 4789.4－2010进行血清分型，并采用K-B纸片琼脂扩散法测定分离株耐药情况。结果表明，共检出阳性样品56份，阳性率为7.9%，其中52份为动物性原料；经血清分型得到阿贡纳沙门氏菌（Agona）5株，阿贡纳沙门氏菌Ⅱ（AgonaⅡ）1株，博内茅斯（Bournemouth）1株和摩科拉（Mokola）1株，其余菌株因血清种类有限，仅分型至群，未能进一步分型；药敏试验结果显示，饲料中沙门氏菌耐药率比人源和动物源性沙门氏菌低，但仍分离到13重耐药株1株，4重耐药株2株，3重耐药株9株，2重耐药株12株，且各菌株耐药谱不同。饲料及其原料中沙门氏菌检出率和耐药性相对较低，但仍存在个别菌株耐药情况严重的现象，有必要继续加大、加强针对抗生素使用的监管力度。
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| 关 键 词： | 沙门氏菌 血清分型 耐药分析 |
| 收稿时间： | 2013-05-15 |
Identification and Antibiotic Analysis of Salmonella Isolated from Feed and its Original Materials |
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| SHE Rong,LIU Yao-min,DU Xue-li,YAN Qi-gui.Identification and Antibiotic Analysis of Salmonella Isolated from Feed and its Original Materials[J].China Animal Husbandry & Veterinary Medicine,2013,40(12):204-207. |
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| Authors: | SHE Rong LIU Yao-min DU Xue-li YAN Qi-gui |
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| Institution: | 1. Inspection Center, Tongwei Co., Ltd., Chengdu 610041, China;2. College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China;3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China |
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| Abstract: | To establish the theoretical basis for the prevention and control of Salmonella spreading, the experiment was to analyze the contamination and drug resistance of Salmonella in feed and its original materials. 707 samples were detected and Salmonella were isolated and identified in accordance with the methods GB/T 13091－2002, the serotype was classified in accordance with GB 4789.4－2010, and Kirby-Bauer disc diffusion method was used to detect the resistance of strains. The results showed that fifty-six (7.9%) strains were demonstated as positive, 52 of which were from the materials of animal origin. Four serotypes were found, including Agona, AgonaⅡ, Bournemouth, Mokola. By drug susceptibility test, the antibiotic rate of Salmonella in feed was much lower than those originated from human and animals, the rest of strains due to the categeries limitaion of serum, the serotype only classified into the group, failed to further classification. Drug sensitive test showed that there were still one strain of 13 multidrug-resistant, two strains of 4 multidrug-resistant, twelve strains of 2 multidrug-resistant and nine strains of 3 multidrug-resistant with different resistance spectrum. Although the test demonstrated relatively low percentage and level of Salmonella in the feed, there still had a few individual strains with serious drug resistance, so it was necessary to watch the application of antibiotics in animal husbandry strictly. |
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| Keywords: | Salmonella serotype drug-resistance |
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