| 动物源产CMY-2大肠杆菌分子流行病学研究 |
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| 引用本文： | 郭玉芳,赵秋云,姜芮,任金程,杨玲,甄盼盼,汤电,蒋红霞.动物源产CMY-2大肠杆菌分子流行病学研究[J].中国畜牧兽医,2013,40(10):47-51. |
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| 作者姓名： | 郭玉芳 赵秋云 姜芮 任金程 杨玲 甄盼盼 汤电 蒋红霞 |
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| 作者单位： | 1. 华南农业大学兽医学院, 广东省兽药研制与安全评价重点实验室, 广东广州 510642;2. 河北新华科极兽药有限公司, 河北石家庄 050000;3. 武汉科前动物生物制品有限责任公司, 湖北武汉 430000 |
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| 基金项目： | 国家自然科学基金—广东基金联合基金重点项目(U1031004)。 |
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| 摘 要： | 为调查产CMY-2大肠杆菌在广东各养殖场的流行情况,对2010—2011年间分离自猪、鸡、鸭、鹅等动物的1293株大肠杆菌,采用PCR方法筛选出blaCMY-2阳性菌株,琼脂稀释法测定阳性菌株对17种抗微生物药物的敏感性;接合转移试验和XbaⅠ酶切PFGE图谱分析blaCMY-2基因转移扩散的方式。结果显示,1293株大肠杆菌中27 株含有blaCMY-2 基因,检出率为2.09%,均为多重耐药菌株,主要耐药谱型为AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX;27 株携带blaCMY-2 基因菌株中有14 株的blaCMY-2 基因可随质粒转移到受体菌E.coli C600中,且往往与blaTEM-1和(或)qnrS1共同转移;PFGE分析结果显示,27 株携带blaCMY-2 基因菌株共产生17条谱带,其中有4株菌株,两两分别来自同一地区,存在克隆传播关系。提示,在广东地区食品动物养殖场内存在产CMY-2大肠杆菌的克隆传播,且blaCMY-2 基因伴随可转移质粒或其他可转移移动元件可能是造成产CMY-2大肠杆菌流行分布的主要原因。
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| 关 键 词： | 大肠杆菌 耐药性 动物源 CMY-2 |
| 收稿时间： | 2013-03-20 |
Epidemiology Investigation of CMY-2-producing E.coli from Animals |
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| GUO Yu-fang,ZHAO Qiu-yun,JIANG Rui,REN Jin-cheng,YANG Ling,ZHEN Pan-pan,TANG Dian,JIANG Hong-xia.Epidemiology Investigation of CMY-2-producing E.coli from Animals[J].China Animal Husbandry & Veterinary Medicine,2013,40(10):47-51. |
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| Authors: | GUO Yu-fang ZHAO Qiu-yun JIANG Rui REN Jin-cheng YANG Ling ZHEN Pan-pan TANG Dian JIANG Hong-xia |
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| Institution: | 1. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Hebei Xinhua Keji Animal Medicine Co., Ltd., Shijiazhuang 050000, China;3. Wuhan Keqian Animal Biological Products Co., Ltd., Wuhan 430000, China |
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| Abstract: | To investigate the epidemiology of CMY-2-producing E.coli from different animals throughout Guangdong province, 1293 isolates from pig, chicken, duck and goose from 2010 to 2011 were recruited in this study. All the isolates were screened for blaCMY-2 gene by PCR and sequenced; the MICs of blaCMY-2-positive isolates to 17 kinds of antimicrobial drugs were tested by agar dilution; conjugation experiments were performed on blaCMY-2 harboring isolates to explore the transferability of blaCMY-2 gene to E.coli C600, and XbaⅠ-PFGE analysis were used to determine the transfer and spread manner of blaCMY-2 among E.coli isolates. The results showed that 27(2.09%)of 1293 isolates carried blaCMY-2,and were present multi-resistant phenotypes, in which the main one was AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX. The blaCMY-2 gene in 14 of 27 isolates transferred successfully to E.coli C600 by plasmids, and most of them co-transferred with blaTEM-1 and/or qnrS1. PFGE profiles showed that 17 different patterns were successfully got, and two from the same place were clonally related as well as another two isolates. CMY-2 encoding gene on a transferability plasmid or carried by other mobile element presumably was the major cause for the epidemiology of CMY-2-producing E.coli among food animals. |
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| Keywords: | E coli resistance animals CMY-2 |
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