| 利用蛋白质生物芯片技术测定原料乳中15种磺胺类药物的残留量 |
| |
| 引用本文： | 姜金斗,张永胜.利用蛋白质生物芯片技术测定原料乳中15种磺胺类药物的残留量[J].中国畜牧兽医,2013,40(Z1):57-61. |
| |
| 作者姓名： | 姜金斗 张永胜 |
| |
| 作者单位： | 1. 农业部乳品质量监督检验测试中心, 黑龙江哈尔滨 150090;2. 北京博欧实德生物技术有限公司, 北京 100107 |
| |
| 摘 要： | 将抗原、抗体生物芯片用于生鲜乳药物残留的检测中目前相关报道较少，现已商品化的芯片主要有各类抗生素芯片组合、驱虫剂芯片组合、生长促进剂组合等。本试验选用其中能同时定量测定15种磺胺组合的芯片进行研究，考察在生鲜乳药物残留检测中该芯片的准确性、精密度等是否能满足检测的要求。结果发现，本方法线性范围为0～20 ng/mL，2个水平的加标（2倍检出限与5或10倍检出限添加量）回收试验其回收率大都在80%～120%之间，其中加入后者回收率优于前者；通过对前一水平加标10次进行测定，结果发现其变异系数大都在5%～15%之间。通过对阴性样品测定全部低于相应的检出限，质控样品测定结果为参考值的80%～110%。表明磺胺生物芯片各项性能指标完全能满足生鲜乳药物残留半定量的快速筛查，为批量样品筛查提供了可靠的技术保证。
|
| 关 键 词： | 蛋白质生物芯片 生鲜乳 磺胺 |
| 收稿时间： | 2013-08-01 |
Determination on 15 Kinds of Sulfa Drug Residues in Raw Milk Using Protein Biochip Technology |
| |
| JIANG Jin-dou,ZHANG Yong-sheng.Determination on 15 Kinds of Sulfa Drug Residues in Raw Milk Using Protein Biochip Technology[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):57-61. |
| |
| Authors: | JIANG Jin-dou ZHANG Yong-sheng |
| |
| Institution: | 1. Ministry of Agricultural Dairy Quality Supervision and Testing Center, Harbin 150090, China;2. Beijing the Biostest Biotechnology Co., Ltd., Beijing 100107, China |
| |
| Abstract: | The antigen,antibody microarray for detection of drug residues in raw milk were currently very few relevant reports,were now commercially available chip portfolio that there were all kinds of antibiotics, insect repellent combination chip,growth-promoting agent and combination. In this experiment,which could simultaneously quantitative determination of 15 kinds of combinations of sulfonamides chip experimental study, drug residues in raw milk in the chip accuracy,precision and so on would be studied. The results showed that the linear range was 0 to 20 ng/mL,two horizontal spiked (2 times the detection limit and 5 or 10 times the detection limit dosage), their recoveries mostly were in the 80% to 120%, which the latter was better than the former, through previous levels spiked 10 times measurement results,the coefficient of variation mostly was in the 5% to 15%. Through the determination of all the negative samples below the respective detection limits,quality control samples were measured to the reference value of 80% to 110%. Prove sulfa biochip could meet the performance indicators of drug residues raw milk semi-quantitative rapid screening,screening for bulk samples to provide a reliable technical guarantee. |
| |
| Keywords: | protein biochip raw milk sulfonamides |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
