| 广州地区犬细小病毒VP2基因的序列分析 |
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| 引用本文： | 许冬蕾,任常宝,张晓战,陈叶,余文兰,葛曾旭,陈瑞爱,唐兆新.广州地区犬细小病毒VP2基因的序列分析[J].中国畜牧兽医,2013,40(12):38-42. |
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| 作者姓名： | 许冬蕾 任常宝 张晓战 陈叶 余文兰 葛曾旭 陈瑞爱 唐兆新 |
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| 作者单位： | 1. 肇庆大华农生物药品有限公司, 广东肇庆 526238;2. 农业部动物疫病防控生物技术与制品创制 重点实验室, 广东肇庆 526238;3. 华南农业大学兽医学院, 广东广州 510642 |
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| 摘 要： | 犬细小病毒病是一种高度接触性传染病，临床上以急性出血性肠炎和心肌炎为特征。为研究广州地区犬细小病毒病的流行亚型，本试验采集2011—2012年间疑似病犬粪便，进行犬细小病毒（canine parvovirus，CPV）分离鉴定，提取病毒基因组进行VP2基因扩增和序列分析，与GenBank中登录的参考毒株进行比对，结果发现，分离的8株CPV中SCAU-5为CPV-2b 亚型，其余为CPV-2a亚型。结果表明，2011—2012年间广州地区的流行毒株以CPV-2a亚型为主。
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| 关 键 词： | 犬细小病毒 VP2基因 序列分析 广州 |
| 收稿时间： | 2013-05-02 |
Sequence Analysis of Canine Parvovirus VP2 Gene in Guangzhou |
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| XU Dong-lei,REN Chang-bao,ZHANG Xiao-zhan,CHEN Ye,YU Wen-lan,GE Zeng-xu,CHEN Rui-ai,TANG Zhao-xin.Sequence Analysis of Canine Parvovirus VP2 Gene in Guangzhou[J].China Animal Husbandry & Veterinary Medicine,2013,40(12):38-42. |
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| Authors: | XU Dong-lei REN Chang-bao ZHANG Xiao-zhan CHEN Ye YU Wen-lan GE Zeng-xu CHEN Rui-ai TANG Zhao-xin |
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| Institution: | 1. Zhaoqing Dahuanong Biology Medcine Co., Ltd., Zhaoqing 526238, China;2. Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention, Ministry of Agriculture, Zhaoqing 526238, China;3. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Canine parvovirus disease was a highly contagious disease and the clinical symptoms were characterized by acute hemorrhagic enteritis and myocarditis. To estimate the prevalence of the disease around Guangzhou, canine parvovirus (CPV) was isolated from suspected feces from 2011 to 2012. Amplification and sequence analysis of VP2 gene showed the antigenic type of 8 CPV strains except SCAU-5 (CPV-2b) belonged to CPV-2a,which indicated that CPV-2a was the predominate type around Guangzhou area from 2011 to 2012. |
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| Keywords: | canine parvovirus VP2 gene sequence analysis Guangzhou |
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