The Effect of Adding Arabogalactan or Ficoll on Freezing Liquid in the Refrigerating of Cattle Biopsied Embryos |
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| Authors: | HAN Wei FENG Chun-tao BI Jiang-hua CHEN Long LIU Xiao PEI Yan YU Wen-li |
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| Institution: | 1. Shijiazhuang Tianquan Elite Dairy Ltd., Shijiazhuang 050051, China;
2. Beijing Anbo Embryo Biotech Center, Beijing 100107, China |
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| Abstract: | Owing to harmed zona pellucida and decreased cell number after sampled, the survival rate of freezed embryos was lower than those from normal ones. The effect of freezed embryos by addition of ficoll and arabinogalactan in freezing liquid was analyzed in this paper. The embryo recoveries,developmental rate after culturing 24 and 48 h of all kinds of refrigerating fluid were compared with control group,and the final result was derived, 0.03 EAS1 was the best refrigerating fluid in arabogalactan series refrigerating fluid, and 0.6 EFS1 was the best refrigerating fluid in ficoll series refrigerating fluid, transplantation results in line with culturing. |
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| Keywords: | sampled embryos arabogalactan ficoll refrigerating fluid |
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