| 肉鸡沙门氏菌的分离鉴定及药敏试验 |
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| 引用本文： | 王傲雪,张凌云,张桉潮,李昆鹏,张秀丽,梁晚枫,张林波.肉鸡沙门氏菌的分离鉴定及药敏试验[J].中国畜牧兽医,2013,40(7):167-171. |
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| 作者姓名： | 王傲雪 张凌云 张桉潮 李昆鹏 张秀丽 梁晚枫 张林波 |
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| 作者单位： | 1. 延边大学, 吉林延吉 133000;2. 吉林农业大学, 吉林长春 130118;3. 吉林正大实业有限公司, 吉林长春 130033;4. 中国农业科学院北京畜牧兽医研究所, 北京 100193 |
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| 摘 要： | 为了解吉林省某地鸡源性沙门氏菌的感染及耐药情况,对该地7个肉鸡场150份病鸡组织样品进行细菌分离,并进行了血凝试验、致病性试验和药敏试验。分离得到26株沙门氏菌,经血凝鉴定有19株呈阳性,其中5株呈强阳性并均能使雏鸡致死。药敏试验结果显示,该5株沙门氏菌对头孢噻肟较为敏感;对氨苄西林、替米考星、林可霉素、阿奇霉素和头孢拉定表现出极高的耐药性,耐药率高达100%;诺氟沙星、恩诺沙星、环丙沙星、强力霉素和氯霉素5种临床用药与痢菌净不具有协同或累加作用。试验结果表明,沙门氏菌是危害该地肉鸡养殖场的主要病原菌之一,且耐药性十分严重。
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| 关 键 词： | 沙门氏菌 分离鉴定 药敏试验 |
| 收稿时间： | 2012-12-25 |
Isolation,Identification and Drug Sensitive Assay of Salmonella From Broiler |
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| WANG Ao-xue,ZHANG Ling-yun,ZHANG An-chao,LI Kun-peng,ZHANG Xiu-li,LIANG Wan-feng,ZHANG Lin-bo.Isolation,Identification and Drug Sensitive Assay of Salmonella From Broiler[J].China Animal Husbandry & Veterinary Medicine,2013,40(7):167-171. |
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| Authors: | WANG Ao-xue ZHANG Ling-yun ZHANG An-chao LI Kun-peng ZHANG Xiu-li LIANG Wan-feng ZHANG Lin-bo |
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| Institution: | 1. Yanbian University, Yanji 133000, China;2. Jilin Agricultural University, Changchun 130118, China;3. Jilin China Tai Enterprise Co., Ltd., Changchun 130033, China;4. Institute of Animal Sciences, China Academy of Agricutural Sciences, Beijing 100193, China |
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| Abstract: | To find out the situations about infection of Salmonella and its drug resistance somewhere in Jilin province,the bacteria were isolated from 150 tissue samples of sick broiler of 7 farms in this city,and hemagglutinin test, pathogenity test and drug sensitive test were carried out. 26 strains were isolated, and 19 strains were identified positive by the HA,of which five were strongly positive and killed broiler, drug sensitive test results showed that the 5 strains of Salmonella were more sensitive to cefotaxime, and very high resistance to ampicillin,tilmicosin,lincomycin,azithromycin and cephradine,resistance rates as high as 100%. Norfloxacin,enrofloxacin,ciprofloxacin,doxycycline and chloramphenicol didn’t have synergistic or additive effect with mequindox which was used in clinical. These results suggested that Salmonella was one of the main pathogens harmful to the broiler of farms, and resistance was very serious. |
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| Keywords: | Salmonella isolation and identification drug sensitive test |
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