| 过滤器辅助样品前处理法测定乳脂肪球膜蛋白质的研究 |
| |
| 引用本文： | 芦晶,刘鹭,张书文,吕加平.过滤器辅助样品前处理法测定乳脂肪球膜蛋白质的研究[J].中国畜牧兽医,2013,40(Z1):93-100. |
| |
| 作者姓名： | 芦晶 刘鹭 张书文 吕加平 |
| |
| 作者单位： | 中国农业科学院农产品加工研究所, 北京 100193 |
| |
| 基金项目： | 国家科技合作与交流专项（010S2012ZR0302）；奶牛产业技术体系北京市创新团队；国家科技支撑计划（2012BAD28B07-3）；国家科技支撑计划（2011BAD09B02）。 |
| |
| 摘 要： | 牛乳是世界上消费最广泛的乳类，并为消费者提供必需的营养物质。牛乳包括蛋白质，脂肪，糖类，维生素和矿物质。蛋白质是牛乳的重要组成部分，包括酪蛋白、乳清蛋白及乳脂肪球膜蛋白质。牛乳乳脂肪球被乳脂肪球膜覆盖，乳脂肪球膜是一个含有3层膜的膜结构，其上附着或者包含蛋白质。近些年来，对于乳中蛋白质的鉴定成为新的研究热点。随着蛋白质组学技术的发展，鉴定出的蛋白质的种类和数量都在增加。但是由于乳脂肪球膜的特殊性质，在牛乳乳脂肪球膜蛋白质的鉴定中，缺乏方便快捷及效果好的方法。作者运用过滤器辅助样品前处理法（FASP）结合纳升液相质谱（NanoLC-MS/MS）测定牛乳乳脂肪球膜蛋白质的种类和数量，共鉴别出169种蛋白质，是目前鉴别数量最大的一种方法。此方法的建立为今后乳脂肪球膜蛋白质研究领域提供一种方便快捷准确的试验工具。
|
| 关 键 词： | 牛乳蛋白 蛋白质组学 过滤器辅助样品前处理 质谱 |
| 收稿时间： | 2013-08-02 |
Use of Filter Aided Sample Preparation to Study Milk Fat Globule Membrane Proteins |
| |
| LU Jing,LIU Lu,ZHANG Shu-wen,LV Jia-ping.Use of Filter Aided Sample Preparation to Study Milk Fat Globule Membrane Proteins[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):93-100. |
| |
| Authors: | LU Jing LIU Lu ZHANG Shu-wen LV Jia-ping |
| |
| Institution: | Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China |
| |
| Abstract: | Bovine milk is consumed widely in the world. It provides multiple nutrients to human, including protein, fat, carbohydrate, vitamins and minerals. Protein is a major component of bovine milk. It contains caseins, whey protein and milk fat globule membrane protein (MFGM). Milk fat exists as milk fat globules in bovine milk and covered by a three-layer membrane which consists of lipids and protein. Recently, the identification of milk proteins gained interests. As the development of proteomic techniques, the number of identified milk protein is increasing. However, no fast and efficient method has been arisen to identify the MFGM proteins because of their hydrophobicity. Filter-aided sample preparation and NanoLC-MS/MS were combined and applied to study MFGM protein in present study. By using these methods, it is now possible to study MFGM in a fast and efficient way. |
| |
| Keywords: | milk proteins proteomics filter-aided sample preparation mass spectrometry |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
