Selection of Reference Genes in Mice Infected with H5N1 Avian Influenza Virus |
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| Authors: | WU Qiao ZHANG Bin TANG Cheng YUE Hua |
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| Institution: | College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | This experiment was conducted to assess the stability of six candidate reference genes (Ywhaz,Hprt1,Sdha,Actb,Ppia and 18S rRNA) in spleen of mice infected with H5N1 avian influenza virus (H5N1 AIV). The mice were infected with the virulent strain A/DK/GD/378/08 of H5N1 AIV and harvested at 12,24,48,72,96,120 and 144 h post-infection. The expression levels of the six reference genes in infected and uninfected mice were determined by Real-time PCR and assessed the stability of reference genes by Genorm software. The results showed that the stability of six reference genes in normal mouse spleen tissue descended as following: Ywhaz/Hprt,Sdha,Actb,Ppia,18S rRNA, the stability of six reference genes in mouse spleen tissue infected with H5N1 AIV descended as following: Ppia/Sdha,18S rRNA,Hprt,Ywhaz,Actb. Furthermore,based on comprehensive evaluation of the normal and the infected H5N1 AIV,the stability of six reference genes in mouse spleen tissue descended as following: Sdha/Hprt1,Ywhaz,Ppia,Actb,18S rRNA. Therefore,the Ppia and Sdha were the best reference genes to use in the study of replication levels of H5N1 AIV in spleen of mice. However,for the study of organism response to H5N1 AIV infection,the Sdha and Hprt1 were the best reference genes. |
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| Keywords: | H5N1 avian influenza virus mice reference genes Real-time PCR |
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