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Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room
Authors:MIAO Hai-sheng  LI Le  ZHU Jian-bo  XIAO Lei  LI Hua-chun
Institution:Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
Abstract:In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue samples which were kept in 4℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.
Keywords:blue-tongue virus  storage in cold room  stability  
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