| 超高效液相色谱―串联质谱法测定饲料中的万古霉素 |
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| 引用本文： | 彭丽,班付国,张发旺,吴宁鹏,李慧素.超高效液相色谱―串联质谱法测定饲料中的万古霉素[J].中国畜牧兽医,2013,40(11):74-78. |
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| 作者姓名： | 彭丽 班付国 张发旺 吴宁鹏 李慧素 |
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| 作者单位： | 河南省兽药监察所, 河南郑州 450008 |
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| 摘 要： | 试验旨在建立饲料中万古霉素的超高效液相色谱—串联质谱检测方法,以0.05 mol/L磷酸盐缓冲液—乙腈(85:15,V/V)提取样品中的万古霉素,提取液经过强阳离子交换固相萃取柱净化,采用液相色谱—串联质谱多反应监测模式进行测定,外标法定量。不同饲料基质中,万古霉素在25~1000 ng/mL浓度范围内呈良好的线性关系。本方法对万古霉素在饲料中的检测限为0.05 mg/kg,定量限为0.1 mg/kg。在不同饲料中添加浓度范围为0.1~100 mg/kg,其平均回收率为86.7%~117.1%,批内变异系数在1.0%~17.4%之间,批间变异系数在2.9%~10.1%之间。本方法分析速度快、灵敏度高、重现性好,各项技术指标均满足国内外相关法规要求,可用于饲料中万古霉素含量的测定。
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| 关 键 词： | 饲料 万古霉素 超高效液相色谱—串联质谱法 |
| 收稿时间： | 2013-04-01 |
Determination of Vancomycin in Feeds by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| PENG Li,BAN Fu-guo,ZHANG Fa-wang,WU Ning-peng,LI Hui-su.Determination of Vancomycin in Feeds by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J].China Animal Husbandry & Veterinary Medicine,2013,40(11):74-78. |
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| Authors: | PENG Li BAN Fu-guo ZHANG Fa-wang WU Ning-peng LI Hui-su |
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| Institution: | Henan Institute of Veterinary Drug Control, Zhengzhou 450008, China |
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| Abstract: | A method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) had been developed for the determination of vancomycin in feeds. The analyte was extracted with phosphate buffer -acetonitrile(85:15,V/V),and cleaned by strong cation exchange solid phase extraction cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring (MRM) using external standard method. The calibration curves of vancomycin were linear over the concentration ranges of 25 to 1000 ng/mL in different matrix. Detection and quantification limits of the method for the analyte were 0.05 and 0.1 mg/kg in feeds. The average recoveries ranged from 86.7% to 117.1% at the spiked level of 0.1 to 100 mg/kg in feeds. The inter-run and between-run relative standard deviation was 1.0% to 17.4% and 2.9% to 10.1%,respectively. The method was reliable,sensitive and reproducibility,its performance could meet the requirements of the domestic and international legislation.The method adapted to the determination of vancomycin in feeds. |
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| Keywords: | feeds vancomycin UPLC-MS/MS |
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