| 荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究 |
| |
| 引用本文： | 李晓霞,汪浩,曹平华,禹学礼,栗颖华.荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究[J].中国畜牧兽医,2013,40(6):161-164. |
| |
| 作者姓名： | 李晓霞 汪浩 曹平华 禹学礼 栗颖华 |
| |
| 作者单位： | 1. 河南科技大学动物科技学院, 河南洛阳 471003;2. 河南农业经济学校, 河南洛阳 471002 |
| |
| 基金项目： | 河南科技大学博士科研启动基金(09001551);国家自然科学基金(31201801;31172207)。 |
| |
| 摘 要： | 为探讨荷斯坦牛和西门塔尔牛冻精的精液品质及体外受精后胚胎发育能力的差异,利用目测法、低渗膨胀法和考马斯亮蓝染色法评估了荷斯坦牛和西门塔尔牛冻精的活力、质膜完整率和顶体完整率,并比较了二者冻精体外受精后胚胎的卵裂率和囊胚率。结果表明,荷斯坦牛和西门塔尔牛冻精的活力(30.4%和27.2%)、质膜完整率(41.96%和36.22%)和顶体完整率(77.02%和73.02%)均无显著差异(P>0.05),但荷斯坦牛冻精体外受精后的卵裂率(57.5%和48.6%)和囊胚率(30.3%和23.2%)显著高于西门塔尔牛冻精(P<0.05)。提示,不同品种公牛精液体外受精后的发育能力有显著差异(P>0.05)。
|
| 关 键 词： | 荷斯坦牛 西门塔尔牛 精液品质 体外受精 |
| 收稿时间： | 2013-01-18 |
Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls |
| |
| LI Xiao-xia,WANG Hao,CAO Ping-hua,YU Xue-li,LI Ying-hua.Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls[J].China Animal Husbandry & Veterinary Medicine,2013,40(6):161-164. |
| |
| Authors: | LI Xiao-xia WANG Hao CAO Ping-hua YU Xue-li LI Ying-hua |
| |
| Institution: | 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;2. Henan Agroeconomical School, Luoyang 471002, China |
| |
| Abstract: | The objectives of this study were to investigate differences in semen quality and development of embryos after in vitro fertilization with frozen-thawed sperm from either Holstein or Simmental bulls. Progressive motility, plasma membrane integrity and acrosome integrity rates of of frozen-thawed sperm from Holstein and Simmental bulls were assessed using visual observation, the hypoosmotic swelling test and Coomassie Blue staining method, and compared the cleavage and blastocyst rates after IVM/IVF with sperm of Holstein and Simmental bulls. The results showed that progressive motility (30.4% and 27.2%),plasma membrane integrity (41.96% and 36.22%) and acrosome integrity rates (77.02% and 73.02%) of frozen-thawed sperm from Holstein and Simmental bulls were all no significant differences (P>0.05), the cleavage (57.5% and 48.6%) and blastocyst rates (30.3% and 23.2%) after IVM/IVF with sperm of Holstein bulls resulted in significant improvement over sperm of Simmental bulls (P<0.05). It was concluded that there were significant differences on embryo developmental competence of in vitro fertilization using different bull semen. |
| |
| Keywords: | Holstein bull Simmental bull semen quality in vitro fertilization |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
