| 中国对虾抗病性状遗传标记筛选及遗传多样性分析 |
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| 作者姓名： | 李素红 张天时 孔杰 孟宪红 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,青岛,266071;青岛金海力水产科技有限公司,266071
2. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,青岛,266071 |
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| 基金项目： | 国家科技部资助项目，山东省自然科学基金 |
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| 摘 要： | 对人工选育的29个中国对虾半同胞家系进行人工感染WSSV后,选取存活时间最长和存活时间最短各29个个体,组成抗病群体和感病群体。用400个随机引物进行RAPD分析,得到与抗病正相关的特异性遗传标记5个,与抗病负相关的特异性遗传标记两个。15个随机引物在两个群体的扩增位点进行统计,共得到82个位点,其中多态位点34个,占41.46%,两个群体的多态位点比例分别为40.24%和37.8%。Shannon′s多样性指数估算两个群体的平均遗传多态度为0.2177,SLT群体的遗传多态度为0.2190略高于SST群体0.2163;群体内的遗传变异（HPOP/HSP）0.9645,可见96.45%的遗传变异来自于群体内,3.55%的变异来源于群体间。统计各座位的基因频率计算出两个群体的遗传分化指数为0.0294,表明两个群体并没有发生明显的遗传分化。
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| 关 键 词： | 中国对虾 遗传标记 WSSV RAPD |
| 收稿时间： | 2008/1/8 0:00:00 |
| 修稿时间： | 2008/3/28 0:00:00 |
Screen of white-spot-syndrome-virus(WSSV)-resistance molecular markers and analysis of genetic diversity in Fenneropenaeus chinensis |
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| Authors: | LI Su-hong ZHANG Tian-shi KONG Jie MENG Xian-hong |
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| Institution: | 1 Key Laboratory for Sustainable Utilization of Marine Fisheries Resource;Ministry of Agriculture;Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 Qingdao Jin Haili Fisheries Technology Co.;Ltd;266071 |
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| Abstract: | 29 sib-families of Fenneropenaeus chinensis which were obtained by selective breeding were artificially infected with WSSV.29 samples with the longest survival time composed the WSSV-resistant group and 29 samples with the shortest survival time composed WSSV-susceptible group.Random amplified polymorphic DNA(RAPD)technique was employed to analyze these samples.The results were that 5 distinctive genetic markers with disease resistant trait and 2 distinctive genetic markers with disease susceptible trait we... |
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| Keywords: | WSSV RAPD |
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