| 猪10号染色体上影响血常规指标的数量性状基因座(QTL)定位 |
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| 引用本文： | 张陈华,蔡绍倩,巩元芳,卢昕,殷宗俊,张勤.猪10号染色体上影响血常规指标的数量性状基因座(QTL)定位[J].中国农业科学,2011,44(2):395-401. |
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| 作者姓名： | 张陈华 蔡绍倩 巩元芳 卢昕 殷宗俊 张勤 |
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| 作者单位： | (安徽农业大学动物科技学院) |
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| 基金项目： | 国家重点基础研究发展计划(2006CB102104); 安徽省科技攻关项目(07010302136、08010301079); 安徽省教育厅自然科学基金重点项目(KJ2009A114) |
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| 摘 要： | 【目的】检测定位猪10号染色体上影响血常规指标的数量性状位点。【方法】以3个品种(大白猪、长白猪、松辽黑猪)16个公猪家系共计368头试验猪组成资源群体,在猪10号染色体上共选取13个微卫星标记,采用基于线性混合模型方法,对影响与猪白细胞、红细胞和血小板相关的共计18项血常规指标的数量性状基因座(quantitative trait loci,QTL)进行了检测。通过似然比检验,利用置换法确定显著性阈值。【结果】()13个标记在群体中绝大多数的微卫星属于中度多态的遗传标记,所有微卫星标记在3个品种中的平均等位基因数为3.1754,平均杂合度为0.5215,平均多态信息含量为0.5999,平均香浓指数为1.3222。(2)达到了染色体极显著水平的3个QTL(P＜0.01),分别是影响着红细胞压积(HCT)、血红蛋白含量(HGB)和平均红细胞体积(MCV),影响血小板总数(PLT)的QTL也达到染色体显著水平(P＜0.05)。【结论】定位的4个影响猪血常规的QTL集中在10号染色体81—136cM区域,临近的标记分别为SW249、SWR136、S0070和SW1894。
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| 关 键 词： | 猪 微卫星 QTL定位 血常规指标 |
| 收稿时间： | 2010-05-04; |
Mapping of Quantitative Trait Loci Affecting Haematological Traits on Swine Chromosome 10 |
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| ZHANG Chen-hua,CAI Shao-qin,GONG Yuan-fang,LU Xin,YIN Zong-jun,ZHANG Qin.Mapping of Quantitative Trait Loci Affecting Haematological Traits on Swine Chromosome 10[J].Scientia Agricultura Sinica,2011,44(2):395-401. |
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| Authors: | ZHANG Chen-hua CAI Shao-qin GONG Yuan-fang LU Xin YIN Zong-jun ZHANG Qin |
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| Institution: | ZHANG Chen-hua1,CAI Shao-qin1,GONG Yuan-fang2,LU Xin2,YIN Zong-jun1,ZHANG Qin2(1College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,2College of Animal Science and Technology,China Agricultural University/Key Laboratory Animal Genetics and Breeding of the Ministry of Agricultrual,Beijing 100193) |
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| Abstract: | 【Objective】 The study was performed for detecting and mapping quantitative trait loci (QTL) affecting haematological traits on the swine chromosome 10. 【Method】 A pig resource population consisted 368 individuals of three breeds (Landrace, Large White and Songliao Black Pig) distributed in 16 boar families. Thirteen microsatellite markers on swine chromosome 10 were selected and a linear mixed model was used to detect quantitative trait loci (QTL) affecting 18 haematological traits including white blood cell count (WBC), and blood cell (RBC), and blood platelet counts (PLT). Then, the method of substitution was used to calculate significant threshold values by likelihood ratio test. 【Result】 Thirteen microsatellite markers of this population were the genetic markers of medium polymorphism. On average, the number of alleles was 3.1754, heterozygosity was 0.5215, and polymorphic information content was 0.5999, shannon index was 1.3222, respectively, during all microsatellite markers of three breeds. Four significant QTL (P＜0.05) were identified on chromosome 10 affecting HCT, HGB, MCV and PLT, respectively. 【Conclusion】 Four QTLs affecting haematological traits were found to be located at 81-136 cM on chromosome 10. The markers close by were SW249, SWR136, S0070, and SW1894. |
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| Keywords: | swine microsatellite QTL mapping haematological traits |
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