| 不同体细胞数牛乳中乳蛋白的比较蛋白质组学研究 |
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| 引用本文： | 杨永新,王加启,卜登攀,李珊珊,周凌云.不同体细胞数牛乳中乳蛋白的比较蛋白质组学研究[J].中国农业科学,2011,44(12):2545-2552. |
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| 作者姓名： | 杨永新 王加启 卜登攀 李珊珊 周凌云 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室 |
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| 基金项目： | 国家“973”计划项目(2011CB100805) |
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| 摘 要： | 【目的】分析不同体细胞数牛乳中乳蛋白的表达变化。【方法】采用二维凝胶电泳技术分离了低体细胞数、中体细胞数、高体细胞数和临床乳房炎的牛乳蛋白，考马斯亮蓝G-250溶液染色，液相色谱串联质谱检测表达变化的蛋白点。【结果】在中体细胞数和高体细胞数乳中酪蛋白水解产物和白蛋白增加，在临床乳房炎牛乳中 α-、β-和κ-酪蛋白几乎全部水解，而白蛋白、转铁蛋白、纤维蛋白原β链和结合珠蛋白等乳清蛋白表达量增加。【结论】展示了不同体细胞数牛乳中乳蛋白的凝胶表达图谱，同时表明牛乳酪蛋白的水解程度随着体细胞数的增加而增加，至严重临床乳房炎时牛乳中酪蛋白几乎全部水解而乳清蛋白急剧增加。
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| 关 键 词： | 奶牛 体细胞数 乳蛋白 质谱检测 |
| 收稿时间： | 2010-07-09; |
Comparative Proteomic Analysis of the Changes of Milk Protein Associated with Somatic Cell Counts |
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| YANG Yong-xin,WANG Jia-qi,BU Deng-pan,LI Shan-shan,ZHOU Ling-yun.Comparative Proteomic Analysis of the Changes of Milk Protein Associated with Somatic Cell Counts[J].Scientia Agricultura Sinica,2011,44(12):2545-2552. |
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| Authors: | YANG Yong-xin WANG Jia-qi BU Deng-pan LI Shan-shan ZHOU Ling-yun |
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| Institution: | YANG Yong-xin1,2,WANG Jia-qi1,BU Deng-pan1,LI Shan-shan1,ZHOU Ling-yun1(1Institute of Animal Science,Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Nutrition,Beijing 100193,2Institute of Animal Science,Anhui Academy of Agricultural Sciences,Hefei 230031) |
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| Abstract: | 【Objective】The objective of the study is to investigate the changes of the milk protein with low somatic cell counts, medium somatic cell counts, high somatic cell counts and clinical mastitis.【Method】Milk proteins were examined using an integrated proteomic approaches consisting of protein separation by two-dimensional gel electrophoresis, stained with Coomassie Blue G-250 solution, and protein identification by HPLC coupled with tandem ion trap spectrometry.【Result】Albumin and caseins variant were increased in milk with medium somatic cell counts, high somatic cell counts and clinical mastitis comparated with low somatic cell counts. An abundant caseins (α-, β- and κ-caseins) were degraded, while low abundant protein serum albumin, transferrin, fibrinogen beta chain, and haptoglobin could be identified in clinical mastitic milk.【Conclusion】Results suggested that caseins were degraded in milk with medium somatic cell counts, and significantly increased in high somatic cell counts, and abruptly increased in clinical mastitis. The findings may provide a survey of milk protein modulation and increase the knowledge of proteomic profile of milk protein changes with low somatic cell counts, medium somatic cell counts, high somatic cell counts and clinical mastitis to improve the technological quality of milk. |
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| Keywords: | dairy cows somatic cell counts milk proteome mass spectroscopy |
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