| 水稻骨干亲本不同发育时期蛋白质表达的多态性 |
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| 引用本文： | 王宪云,刘钊,曹英豪,白辉,刘丽娟,李莉云,刘国振.水稻骨干亲本不同发育时期蛋白质表达的多态性[J].中国农业科学,2011,44(14):2849-2856. |
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| 作者姓名： | 王宪云 刘钊 曹英豪 白辉 刘丽娟 李莉云 刘国振 |
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| 作者单位： | 河北农业大学生命科学学院 |
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| 基金项目： | 国家“973”基础研究项目(2006CB101705) |
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| 摘 要： | 【目的】通过对水稻骨干亲本在不同发育时期的蛋白质表达特征研究，了解其形成机理并促进其应用。【方法】采用Western blotting调查了6个水稻骨干亲本（9311、培矮64s、特青、珍汕97B、广陆矮4号和矮脚南特）在不同生长发育时期/部位（苗期地上部与地下部、开花期剑叶和穗子、成熟期剑叶和穗子）与光合作用、活性氧清除及胁迫防御相关的9个蛋白质的表达。【结果】9个蛋白质的表达在不同骨干亲本间有多态性，尤其在地下部和穗子等部位的表达差异较大，而在叶片中的表达比较接近；此外，蛋白质表达在不同生长发育时期/部位间也有多态性，反映了蛋白质表达的时空特异性。【结论】初步建立一条利用蛋白质特异抗体了解水稻蛋白质表达的研究途径。
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| 关 键 词： | 水稻 骨干亲本 蛋白质 多态性 |
| 收稿时间： | 2010-12-07; |
The Profiling of Protein Expression at Different Developmental Stages of Elite Rice Varieties |
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| WANG Xian-yun,LIU Zhao,CAO Ying-hao,BAI Hui,LIU Li-juan,LI Li-yun,LIU Guo-zhen.The Profiling of Protein Expression at Different Developmental Stages of Elite Rice Varieties[J].Scientia Agricultura Sinica,2011,44(14):2849-2856. |
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| Authors: | WANG Xian-yun LIU Zhao CAO Ying-hao BAI Hui LIU Li-juan LI Li-yun LIU Guo-zhen |
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| Institution: | WANG Xian-yun1,LIU Zhao1,CAO Ying-hao1,2,BAI Hui2,LIU Li-juan1,LI Li-yun1,LIU Guo-zhen1(1College of Life Sciences,Agricultural University of Hebei,Baoding 071001,Hebei,2Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 101300) |
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| Abstract: | 【Objective】 The characterization of protein expression at different developmental stages may help to understand the forming mechanisms of elite varieties and then promote their applications in breeding.【Method】In this study, Western blotting were used to investigate the expression of nine proteins, in the categories of photosynthesis, reactive oxygen scavenging, and defense/stress responses, among six rice elite varieties including 9311, PA64s, Teqing, Zhenshan 97B, Guangluai 4 and Aijiaonante, at different developmental stages/tissues including the shoot and root at seedling stage, the flag leaf and panicle at &;#64258;owering stage, the flag leaf and panicle at &;#64257;lling stage.【Result】The results indicated that polymorphisms of protein expression presented among different rice varieties, the expression patterns in root and panicle varied to a large extent, while the expression in leaves was similar among different rice varieties. Additionally, the polymorphisms also presented among different developmental stages/tissues, indicating the temporal and spatials expressions of these proteins.【Conclusion】This study has established a strategy to investigate the expression of rice proteins by using antibodies. |
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| Keywords: | rice(Oryza sativa L ) elite variety protein polymorphism |
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