| 粉女郎红掌组培快繁技术研究 |
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| 引用本文： | 陈华,吴子平.粉女郎红掌组培快繁技术研究[J].安徽农学通报,2010,16(21):55-57. |
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| 作者姓名： | 陈华 吴子平 |
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| 作者单位： | 泉州市洛江区罗溪镇政府,福建泉州,362015;泉州市洛江区生产力促进中心,福建泉州,362011 |
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| 摘 要： | 以粉女郎红掌的茎、叶、叶柄作为研究对象,进行组培快繁技术研究,结果表明：茎、叶、叶柄的最佳消毒条件分别为0.1%升汞消毒15-20m in、5-7m in、10-12m in,最佳的外植体为叶片;适宜的诱导培养基为：愈伤诱导,MS＋6-BA1.0mg/L＋2,4-D0.1mg/L＋葡萄糖30 g/L＋卡拉胶6 g/L;增殖诱导,MS＋6-BA3.0mg/L＋NAA0.01mg/L;壮苗生根,MS＋活性碳。其中2,4-D对愈伤诱导比较敏感,前期为良好的促进作用,后期表现为杀灭作用。壮苗生根过程添加土豆汁、香蕉汁容易滋生细菌。
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| 关 键 词： | 粉女郎红掌 组培快繁 技术 |
Study on Tissue Culture of Anthurium 'Pink Lass' |
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| ChenHua et al..Study on Tissue Culture of Anthurium 'Pink Lass'[J].Auhui Agricultural Science Bulletin,2010,16(21):55-57. |
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| Authors: | ChenHua |
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| Institution: | ChenHua1 et al.(Quanzhou LuoXi luojiang district government,Quanzhou 362015,China) |
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| Abstract: | The research reports on tissue culture in Anthurium 'Pink Lass'.,The suitable sterilized time is 15~20min to stems,5 ~7min to leaves,10~12min to petioles,and the leaf is the fitting explant.Mediums are made of MS +6-BA1.0 mg/L+2,4-D0.1mg/L+sugar30g/L+agar 6g/L for callus induction,the rate of callus induction is up to 100;MS +6-BA3.0 mg/L+NAA0.01mg/L for multiplication,the propagation coefficient is up to 6.25;MS +AC for rooting,and the conforming rate is up to 90%.The callus is sensitive to 2,4-D that prom... |
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| Keywords: | Anthurium 'Pink Lass' Tissue culture Technique |
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