| 支撑物对试管内蓝莓组培苗生根的影响 |
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| 引用本文： | 郭茹,孙世海,叶霞,华仁锐.支撑物对试管内蓝莓组培苗生根的影响[J].防护林科技,2020(1):25-27,45. |
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| 作者姓名： | 郭茹 孙世海 叶霞 华仁锐 |
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| 作者单位： | ;1.天津农学院 |
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| 基金项目： | 天津农学院研究生培养质量提升项目(2017YPY006)。 |
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| 摘 要： | 以蓝莓品种‘蓝丰’组培苗为材料,采用正交试验设计,研究了不同类型的支撑物(珍珠岩、草炭和琼脂)、不同液体培养基(WPM、1/2WPM和1/4WPM)、不同激素(IBA、NAA、IBA+NAA)及其不同添加浓度(0.5 mg·L-1、1 mg·L-1、2 mg·L-1)对试管内蓝莓组培苗生根的影响。结果显示:以珍珠岩为支撑物的各处理有效生根率分别为82.5%、77.5%和79.31%,明显高于其他处理,且根系发达,分支多。基础液体培养基以WPM的R值最大,为44.44;激素类型以0.5 mg·L-1 IBA+0.5 mg·L-1 NAA组合的R值最大,为42.22;激素浓度以1 mg·L-1的R值最大,为43.33。蓝莓组培苗的最佳生根组合方式为以珍珠岩为支撑物,添加0.5 mg·L-1 IBA和0.5 mg·L-1 NAA的WPM液体培养基。
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| 关 键 词： | 蓝莓 组织培养 生根 正交试验 |
Effect of Support on Root of Vaccinium uliginosum Tissue Culture Seedlings in Test Tubes |
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| Guo Ru,Sun Shihai,Ye Xia,Hua Renrui.Effect of Support on Root of Vaccinium uliginosum Tissue Culture Seedlings in Test Tubes[J].Protection Forest Science and Technology,2020(1):25-27,45. |
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| Authors: | Guo Ru Sun Shihai Ye Xia Hua Renrui |
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| Institution: | (College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin,300384,China) |
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| Abstract: | Theseedlings of Vaccinium uliginosum ’Lanfeng’ in vitro were taken as experimental materials, and the effect of the different supports(perlite, peat, agar), differentliquid medium(WPM, 1/2 WPM and 1/4 WPM), different hormones(IBA, NAA and IBA+NAA)and their concentrations(0.5 mg·L-1, 1 mg·L-1 and 2 mg·L-1) on the rooting of Blueberry seedlings in vitro by orthogonal design. Result shows that the effective rooting rates is 82.5%, 77.5% & 79.31% respectively when the perlite be used as a support, and which were obviously higher than other treatments and their seedlings’ root system was developed and the branches were more. The treatment of 0.5 mg·L-1IBA+0.5 mg·L-1NAA and the hormone concentration of 1 mg·L-1 have the maximum R value, which is 42.22, 43.33 respectively. The optimum medium for rooting of seedlings of Vaccinium uliginosum ’Lanfeng’ in vitrois WPM(which being used with liquid culture medium)+the perlite(which being used as the support) + IBA 0.5 mg·L-1+NAA 0.5 mg·L-1. |
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| Keywords: | Vaccinium uliginosum tissue culture root orthogonal experiment |
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