| 灵芝超声波循环提取物成分及活性物质 |
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| 引用本文： | 陈体强,吴岩斌,毛方华,王宏雨.灵芝超声波循环提取物成分及活性物质[J].中国食用菌,2012,31(3):47-49. |
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| 作者姓名： | 陈体强 吴岩斌 毛方华 王宏雨 |
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| 作者单位： | 1. 福建省农业科学院食用菌研究所,福建福州,350014
2. 福建中医药大学,福建福州,350108 |
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| 基金项目： | 福建省公益类科研院所基本科研专项（2009R10038-4）、福建省农业科学院创新团队重点项目（STIFOY08-6） |
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| 摘 要： | 采用超声波循环提取获得一种新的灵芝提取物。分析表明,其蛋白质34.41 g.100-1.g-1、可溶性粗多糖40.0 g.100-1.g-1和矿质元素磷1.66 mg.g-1的含量分别高于普通灵芝提取物32.96%、29.45%和30.71%。提取物中均含有18种氨基酸,全氨基酸总量为17.43 g.100-1.g-1,较普通提取物12.95 g.100-1.g-1高34.57%;此外,还检出2种天然活性成分--γ-氨基丁酸和牛磺酸,含量分别为21.92 mg.100-1.g-1和45.97 mg.100-1.g-1。液相质谱分析结果:从提取物中分离检测到至少13个清晰的分子离子峰,m/z范围为266.1~678.5,与香豆素、甾醇类、三萜酸及三萜皂苷等小分子天然活性成分的分子量信息相匹配。
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| 关 键 词： | 灵芝 浸膏粉 超声波循环提取 一般化学成分 天然活性成分 |
General Chemical Components and Natural Active Substances in Ultrasonic Circulating Extract Powder of Ganoderma lucidum |
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| CHEN Ti-qiang
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WU Yuan-bin
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MAO Fang-hua
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WANG Hong-yu.General Chemical Components and Natural Active Substances in Ultrasonic Circulating Extract Powder of Ganoderma lucidum[J].Edible Fungi of China,2012,31(3):47-49. |
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| Authors: | CHEN Ti-qiang WU Yuan-bin MAO Fang-hua WANG Hong-yu |
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| Institution: | 1. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2.Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China) |
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| Abstract: | A new extract was prepared by Ultrasonic circulating extraction （UCE） , with Ultra-fine particles of log-culti- vated fruiting bodies as productive material. Analyzed results showed that content of protein, crude polysaccharide and mineral element P in new extract powder were 34.41 g.100-1.g-1, 40.0 g.100-l-g-land 1.66/mg-g-1, respectively, and high- er 32.96%, 29.45% and 30.71% than that in ordinary extract powder. There were eighteen kinds of amino acids in the extract powders, and the contents of total amino acids （TAA） and free amino acids （FAA） were 17.43 g.100-1.g-land 4.28 g.100-1 .g~l, with 53.93% ratio of essential amino acids vs total amino acids （E/T=0.5393） . Moreover, two active amino acids,y/-Amino butyric acid （GABA） and Taurine （Tau） were checked out, with contents of 21.92 mg.100-1 .g- hnd 45.97 mg.100-l .g-1 respectively. At least 13 molecular- ions （m/z： 266.1-678.5） were acquired from the extract powder,that were suited with relative molecular molecules of known active compounds, such as coumarin, sterol, triterpene and triterpenoid saponins, by High-performance liquid chromatography coupled with electrospray ionization mass spec- trometry （HPLC-ESI-MS） |
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| Keywords: | Ganoderma lucidum extract powder Ultrasonic circulating extraction general chemical component activecomponent |
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