| 竹黄多糖液态发酵培养基优化研究 |
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| 引用本文： | 陈佳佳,叶亚新,金琎,李兆兰.竹黄多糖液态发酵培养基优化研究[J].安徽农业科学,2009,37(25):11869-11870. |
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| 作者姓名： | 陈佳佳 叶亚新 金琎 李兆兰 |
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| 作者单位： | 苏州科技学院化学与生物工程学院,江苏苏州,215009;南京大学生命科学学院药物生物技术国家重点实验室,江苏南京,210093 |
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| 摘 要： | 目的]为竹黄多糖的工业化生产提供理论依据。方法]通过组织分离，从野生竹黄子实体中获得能产生竹黄多糖的无性株，然后用其进行液态发酵培养，通过摇瓶正交试验确定其最佳培养基配方。结果]碳源、氮源、生长因子和初始pH值的影响均达显著水平（α=0．05）。其显著性依次为：碳源浓度〉生长因子浓度〉酸碱度〉氮源浓度，在交互作用中只有CGF×CN的影响达显著水平。方差分析结果表明，最佳培养基配方为：蔗糖20g／L＋酵母膏8g／L＋玉米浆2g／L，最佳初始pH值为6．0。当摇瓶装量为50／250ml（V／V）。接种量为10％（V／V），培养温度为28℃，摇床转速为120r／min时，120h后发酵液的竹黄多糖含量为8g／L。结论]该试验初步研究了利用竹黄无性菌株通过液态发酵生产竹黄多糖的培养基组成及培养条件。
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| 关 键 词： | 竹黄菌 竹黄多糖 正交试验 液态发酵 |
Study on the Optimization of Liquid Fermentation Medium of Shiraia bambusicola Polysaccharide |
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| CHEN Jia-jia et al.Study on the Optimization of Liquid Fermentation Medium of Shiraia bambusicola Polysaccharide[J].Journal of Anhui Agricultural Sciences,2009,37(25):11869-11870. |
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| Authors: | CHEN Jia-jia |
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| Institution: | CHEN Jia-jia et al (School of Chemical , Biological Engineering,Suzhou University of Science , Technology,Suzhou,Jiangsu 215009) |
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| Abstract: | Objective] The purpose was to supply theoretical foundation for the industrialized production of Shiraia bambusicola polysaccharide.Method] The asexual producing strains of S.bambusicola polysaccharide were obtained from the fruiting bodies of wild S.bambusicola through tissue isolation.Then they were cultured in liquid medium by fermentation and their optimum medium formula was ascertained through orthogonal shaking flask experiment.Result] The influences of carbon source,nitrogen source,growth factors ... |
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| Keywords: | Bamboo parasitic fungus Shiraia bambusicola polysaccharide Orthogonal test Liquid fermentation |
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